RegulonDB

Gene
Name:	mgrB
Synonym(s):	ECK1824, G7002, b1826, yobG
Genome position(nucleotides):	1908623 <-- 1908766 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	46.53
Reference(s):	[1] Kato A., et al., 1999
External database links:

ASAP:	ABE-0006078
ECHOBASE:	EB4126
ECOLIHUB:	mgrB
OU-MICROARRAY:	b1826
STRING:	511145.b1826
COLOMBOS:	mgrB

Product

Name:

PhoQ kinase inhibitor

Synonym(s):

MgrB, YobG

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol,inner membrane

Molecular weight:

5.552

Isoelectric point:

8.039

Motif(s):

Type	Positions	Sequence
	19 -> 47	LWAQVFNMMCDQDVQFFSGICAINQFIPW
	2 -> 24	KKFRWVVLVVVVLACLLLWAQVF

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		5 - cell processes --> 5.5 - adaptations --> 5.5.4 - pH
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:0016020 - membrane GO:0005886 - plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0005515 - protein binding
	biological_process	GO:0010447 - response to acidic pH GO:0044092 - negative regulation of molecular function GO:0071286 - cellular response to magnesium ion GO:0070298 - negative regulation of phosphorelay signal transduction system

Note(s):

Note(s): ...[more].

Evidence:

[IMP] Inferred from mutant phenotype

Reference(s):

[2] Duval M., et al., 2017
[3] Hamdallah I., et al., 2018
[4] Hobbs EC., et al., 2010
[5] Lippa AM., et al., 2009
[6] Lippa AM., et al., 2012
[7] Miyashiro T., et al., 2007
[8] Rao SD., et al., 2021

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

PRODB:

REFSEQ:

UNIPROT:

Operon

Name:

mgrB

Operon arrangement:

Transcription unit	Promoter
mgrB	mgrBp

Transcriptional Regulation
	Display Regulation
Activated by:	PhoP

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	yobHp4	1908900		forward	nd	[ICWHO]	[9]

Evidence
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Kato A., Tanabe H., Utsumi R., 1999, Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters., J Bacteriol 181(17):5516-20
[2] Duval M., Cossart P., 2017, Small bacterial and phagic proteins: an updated view on a rapidly moving field., Curr Opin Microbiol 39:81-88
[3] Hamdallah I., Torok N., Bischof KM., Majdalani N., Chadalavada S., Mdluli N., Creamer KE., Clark M., Holdener C., Basting PJ., Gottesman S., Slonczewski JL., 2018, Experimental Evolution of Escherichia coli K-12 at High pH and with RpoS Induction., Appl Environ Microbiol 84(15)
[4] Hobbs EC., Astarita JL., Storz G., 2010, Small RNAs and small proteins involved in resistance to cell envelope stress and acid shock in Escherichia coli: analysis of a bar-coded mutant collection., J Bacteriol 192(1):59-67
[5] Lippa AM., Goulian M., 2009, Feedback inhibition in the PhoQ/PhoP signaling system by a membrane peptide., PLoS Genet 5(12):e1000788
[6] Lippa AM., Goulian M., 2012, Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ/PhoP system in Escherichia coli., J Bacteriol 194(6):1457-63
[7] Miyashiro T., Goulian M., 2007, Stimulus-dependent differential regulation in the Escherichia coli PhoQ PhoP system., Proc Natl Acad Sci U S A 104(41):16305-10
[8] Rao SD., Igoshin OA., 2021, Overlaid positive and negative feedback loops shape dynamical properties of PhoPQ two-component system., PLoS Comput Biol 17(1):e1008130
[9] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78