RegulonDB RegulonDB 11.1: Gene Form
   

cmoB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

torZ cmoB cmoA yecN

Gene      
Name: cmoB    Texpresso search in the literature
Synonym(s): ECK1872, G7021, b1871, yecP
Genome position(nucleotides): 1953442 --> 1954413
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.06
External database links:  
ASAP:
 ABE-0006241
ECHOBASE:
 EB3788
ECOLIHUB:
 cmoB
OU-MICROARRAY:
 b1871
STRING:
 511145.b1871
COLOMBOS: cmoB


Product      
Name: tRNA U34 carboxymethyltransferase
Synonym(s): CmoB, YecP
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 37.007
Isoelectric point: 6.59
Motif(s):
 
Type Positions Sequence Comment
7 -> 322 FYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQFEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARK
91 -> 91 K UniProt: Decreases affinity toward Cx-SAM and increases affinity toward SAM. Lack of carboxymethyltransferase activity, but can still produce mho5U..
152 -> 154 DPT UniProt: Carboxy-S-adenosyl-L-methionine binding; Sequence Annotation Type: region of interest.
181 -> 182 IE UniProt: Carboxy-S-adenosyl-L-methionine binding; Sequence Annotation Type: region of interest.
200 -> 200 Y UniProt: Slight decrease in Cx-SAM binding affinity. Does not affect carboxymethyltransferase activity..

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
  2 - information transfer --> 2.2 - RNA related --> 2.2.5 - tRNA
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0032991 - protein-containing complex
molecular_function GO:0016765 - transferase activity, transferring alkyl or aryl (other than methyl) groups
GO:0016740 - transferase activity
GO:0042802 - identical protein binding
biological_process GO:0006400 - tRNA modification
GO:0008033 - tRNA processing
GO:0002098 - tRNA wobble uridine modification
External database links:  
ALPHAFOLD:
 P76291
ECOCYC:
 G7021-MONOMER
ECOLIWIKI:
 b1871
INTERPRO:
 IPR027555
INTERPRO:
 IPR010017
INTERPRO:
 IPR029063
MODBASE:
 P76291
PDB:
 4QNX
PDB:
 4QNU
PDB:
 4QNV
PFAM:
 PF08003
PRIDE:
 P76291
PRODB:
 PRO_000022303
REFSEQ:
 NP_416385
SMR:
 P76291
UNIPROT:
 P76291


Operon      
Name: yecN-cmoAB         
Operon arrangement:
Transcription unit        Promoter
yecN-cmoAB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References



RegulonDB