RegulonDB RegulonDB 11.1: Gene Form
   

tcyJ gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dcyD tcyJ fliZ anti-anti-terminator anti-terminator terminator TSS_2257 TSS_2257 TSS_2256 (cluster) TSS_2256 (cluster) tcyJp tcyJp TSS_2254 TSS_2254 TSS_2253 TSS_2253 TSS_2252 TSS_2252 TSS_2251 TSS_2251 dcyDp dcyDp

Gene      
Name: tcyJ    Texpresso search in the literature
Synonym(s): ECK1919, G7039, b1920, fliY, ssi7, yzzR
Genome position(nucleotides): 1999585 <-- 2000385
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.81
External database links:  
ASAP:
 ABE-0006392
ECHOBASE:
 EB2545
ECOLIHUB:
 fliY
OU-MICROARRAY:
 b1920
STRING:
 511145.b1920
COLOMBOS: tcyJ


Product      
Name: cystine ABC transporter periplasmic binding protein
Synonym(s): FliY, Ssi7, TcyJ, YzzR
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,periplasmic space
Molecular weight: 29.039
Isoelectric point: 6.576
Motif(s):
 
Type Positions Sequence Comment
1 -> 29 MKLAHLGRQALMGVMAVALVAGMSVKSFA
30 -> 266 DEGLLNKVKERGTLLVGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRIDVVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNYEEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEAFSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK UniProt: L-cystine-binding protein TcyJ.
44 -> 261 LVGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRIDVVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNYEEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEAFSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFG

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.p - ABC superfamily, periplasmic binding component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
molecular_function GO:0016597 - amino acid binding
GO:0015276 - ligand-gated ion channel activity
GO:0015184 - L-cystine transmembrane transporter activity
biological_process GO:0006865 - amino acid transport
GO:0006791 - sulfur utilization
GO:0015811 - L-cystine transport
GO:1903712 - cysteine transmembrane transport
GO:0015830 - diaminopimelate transport
Note(s): Note(s): ...[more].
Reference(s): [1] Berger EA., et al., 1972
External database links:  
ALPHAFOLD:
 P0AEM9
DIP:
 DIP-47980N
ECOCYC:
 G7039-MONOMER
ECOLIWIKI:
 b1920
INTERPRO:
 IPR001320
INTERPRO:
 IPR018313
INTERPRO:
 IPR001638
MODBASE:
 P0AEM9
PFAM:
 PF00497
PRIDE:
 P0AEM9
PRODB:
 PRO_000022670
PROSITE:
 PS01039
REFSEQ:
 NP_416430
SMART:
 SM00079
SMART:
 SM00062
SMR:
 P0AEM9
UNIPROT:
 P0AEM9


Operon      
Name: fliAZ-tcyJ         
Operon arrangement:
Transcription unit        Promoter
tcyJ
fliAZY
fliAZY


Transcriptional Regulation      
Display Regulation             
Activated by: CysB, H-NS, FlhDC
Repressed by: SutR, NsrR, IHF, MatA, CsgD


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2251 2000146 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2252 2000154 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2253 2000191 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2254 2000398 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2256 (cluster) 2000468 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2257 2000470 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Berger EA., Heppel LA., 1972, A binding protein involved in the transport of cystine and diaminopimelic acid in Escherichia coli., J Biol Chem 247(23):7684-94

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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