RegulonDB

Gene
Name:	mtfA
Synonym(s):	ECK1972, G7063, b1976, yeeI
Genome position(nucleotides):	2043651 --> 2044448
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	45.86
External database links:

ASAP:	ABE-0006558
ECHOBASE:	EB3159
ECOLIHUB:	mtfA
OU-MICROARRAY:	b1976
STRING:	511145.b1976
COLOMBOS:	mtfA

Product

Name:

Mlc titration factor

Synonym(s):

MtfA, YeeI

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

30.279

Isoelectric point:

4.355

Motif(s):

Type	Positions	Sequence	Comment
	15 -> 243	TALPWQEALSIPLLTCLTEQEQSKLVTLAERFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDEWEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGDRASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSEYFFSAPELFAPRFPSLWQRFCQFYQ

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0008134 - transcription factor binding GO:0004177 - aminopeptidase activity GO:0008237 - metallopeptidase activity
	biological_process	GO:0006508 - proteolysis GO:0043433 - negative regulation of DNA-binding transcription factor activity GO:0051090 - regulation of DNA-binding transcription factor activity

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IDA] Inferred from direct assay
[EXP-IGI] Inferred from genetic interaction
[EXP-IMP] Inferred from mutant phenotype
[EXP-IPI] Inferred from physical interaction

Reference(s):

[1] Becker AK., et al., 2006
[2] Gohler AK., et al., 2012

External database links:

ALPHAFOLD:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

mtfA

Operon arrangement:

Transcription unit	Promoter
mtfA

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2283	2043565	reverse	nd	[RS-EPT-CBR]	[3]
promoter	mtfAp1	2043618	forward	nd	[COMP-AINF]	[4]
promoter	TSS_2284	2044204	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_2285	2044549	forward	nd	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[COMP-AINF] Inferred computationally without human oversight

Reference(s)
[1] Becker AK., Zeppenfeld T., Staab A., Seitz S., Boos W., Morita T., Aiba H., Mahr K., Titgemeyer F., Jahreis K., 2006, YeeI, a Novel Protein Involved in Modulation of the Activity of the Glucose-Phosphotransferase System in Escherichia coli K-12., J Bacteriol 188(15):5439-49
[2] Gohler AK., Staab A., Gabor E., Homann K., Klang E., Kosfeld A., Muus JE., Wulftange JS., Jahreis K., 2012, Characterization of MtfA, a novel regulatory output signal protein of the glucose-phosphotransferase system in Escherichia coli K-12., J Bacteriol 194(5):1024-35
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78