RegulonDB

Gene
Name:	insC3
Synonym(s):	ECK0357, G7077, b1997, yi21-3
Genome position(nucleotides):	2069815 <-- 2070180 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.01
External database links:

ASAP:	ABE-0006631
ECHOBASE:	EB4732
ECOLIHUB:	insC-3
ECOO157CYC:	Z1826
ECOO157CYC:	Z5096
ECOO157CYC:	Z4315
ECOO157CYC:	Z0365
ECOO157CYC:	Z1927
ECOO157CYC:	Z3154
OU-MICROARRAY:	b1997
STRING:	511145.b4272
COLOMBOS:	insC3

Product

Name:

CP4-44 prophage; IS2 insertion element repressor InsA

Synonym(s):

InsC3

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

13.452

Isoelectric point:

10.175

Motif(s):

Type	Positions	Sequence
	8 -> 87	EKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLL

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		8 - extrachromosomal --> 8.3 - transposon related
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0004803 - transposase activity
	biological_process	GO:0006310 - DNA recombination GO:0006313 - transposition, DNA-mediated GO:0032196 - transposition

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

insCD3C3D3

Operon arrangement:

Transcription unit	Promoter
insC3D3CD3

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2306	2067312	reverse	nd	[RS-EPT-CBR]	[1]
promoter	insHp2	2067498	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.98. P-value: 5.222e-5 Read more >	[ICWHO]	[2]
promoter	insHp4	2067511	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 6.919. P-value: 6.656e-6 Read more >	[ICWHO]	[2]
promoter	TSS_2307	2067918	reverse	nd	[RS-EPT-CBR]	[1]
promoter	TSS_2308	2067920	reverse	nd	[RS-EPT-CBR]	[1]
promoter	TSS_2309	2067984	forward	nd	[RS-EPT-CBR]	[1]
promoter	TSS_2310 (cluster)	2070209	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2070208 to 2070209, where the highest frequency is at position 2068232. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[1]
promoter	TSS_2311	2071472	forward	nd	[RS-EPT-CBR]	[1]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)