RegulonDB RegulonDB 11.1: Gene Form
   

galF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

wcaM rfbB galF Cis-reg galFp galFp TSS_2398 TSS_2398 TSS_2397 TSS_2397 TSS_2396 TSS_2396 TSS_2395 TSS_2395 TSS_2394 TSS_2394 TSS_2393 TSS_2393 TSS_2392 TSS_2392 TSS_2391 TSS_2391 TSS_2390 TSS_2390

Gene      
Name: galF    Texpresso search in the literature
Synonym(s): ECK2036, G7093, b2042, wcaN, yefG
Genome position(nucleotides): 2113434 <-- 2114327
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.68
External database links:  
ASAP:
 ABE-0006771
ECHOBASE:
 EB3348
ECOLIHUB:
 galF
OU-MICROARRAY:
 b2042
STRING:
 511145.b2042
COLOMBOS: galF


Product      
Name: UTP:glucose-1-phosphate uridylyltransferase, low activity
Synonym(s): GalF, WcaN, YefG
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 32.829
Isoelectric point: 5.792
Motif(s):
 
Type Positions Sequence Comment
5 -> 275 KAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVK

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.10 - sugar nucleotide biosynthesis, conversions
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016779 - nucleotidyltransferase activity
GO:0030234 - enzyme regulator activity
GO:0016740 - transferase activity
GO:0003983 - UTP:glucose-1-phosphate uridylyltransferase activity
biological_process GO:0009058 - biosynthetic process
GO:0009103 - lipopolysaccharide biosynthetic process
GO:0009225 - nucleotide-sugar metabolic process
GO:0050790 - regulation of catalytic activity
GO:0006011 - UDP-glucose metabolic process
Note(s): Note(s): ...[more].
Evidence: [COMP-HINF-FN-FROM-SEQ] Human inference of function from sequence
Reference(s): [1] Marolda CL., et al., 1996
[2] Rahn A., et al., 2003
[3] Stevenson G., et al., 1996
External database links:  
ALPHAFOLD:
 P0AAB6
DIP:
 DIP-35871N
ECOCYC:
 G7093-MONOMER
ECOLIWIKI:
 b2042
INTERPRO:
 IPR029044
INTERPRO:
 IPR005835
INTERPRO:
 IPR005771
INTERPRO:
 IPR005774
MODBASE:
 P0AAB6
PANTHER:
 PTHR43197
PFAM:
 PF00483
PRIDE:
 P0AAB6
REFSEQ:
 NP_416546
SMR:
 P0AAB6
UNIPROT:
 P0AAB6


Operon      
Name: galF         
Operon arrangement:
Transcription unit        Promoter
galF


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2390 2112120 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2391 2112295 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2392 2113123 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2393 2113226 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2394 2113233 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2395 2113235 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2396 2113809 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2397 2113829 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2398 2113953 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Marolda CL., Valvano MA., 1996, The GalF protein of Escherichia coli is not a UDP-glucose pyrophosphorylase but interacts with the GalU protein possibly to regulate cellular levels of UDP-glucose., Mol Microbiol 22(5):827-40

 [2] Rahn A., Whitfield C., 2003, Transcriptional organization and regulation of the Escherichia coli K30 group 1 capsule biosynthesis (cps) gene cluster., Mol Microbiol 47(4):1045-60

 [3] Stevenson G., Andrianopoulos K., Hobbs M., Reeves PR., 1996, Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid., J Bacteriol 178(16):4885-93

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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