RegulonDB RegulonDB 10.8: Gene Form
   

wcaL gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

wzxC wcaM wcaK wcaL wcaMp1 wcaMp1

Gene      
Name: wcaL    Texpresso search in the literature
Synonym(s): ECK2038, G7095, b2044, yefL
Genome position(nucleotides): 2115907 <-- 2117127 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.87
External database links:  
ASAP:
ABE-0006777
ECHOBASE:
EB2522
MIM:
300818
MIM:
300868
OU-MICROARRAY:
b2044
PortEco:
wcaL
STRING:
511145.b2044
COLOMBOS: wcaL


Product      
Name: putative colanic biosynthesis glycosyl transferase
Synonym(s): WcaL, YefL, orf19
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 45.362
Isoelectric point: 9.531
Motif(s):
 
Type Positions Sequence
126 -> 126 F
129 -> 129 A
15 -> 210 ETFVLNQITAFIDMGFEVEILALQKGDTQNTHAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREVLNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTR
144 -> 144 G
205 -> 205 G

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.2 - colanic acid (M antigen)
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016757 - transferase activity, transferring glycosyl groups
GO:0016740 - transferase activity
biological_process GO:0009103 - lipopolysaccharide biosynthetic process
GO:0046377 - colanic acid metabolic process
GO:0045228 - slime layer polysaccharide biosynthetic process
Note(s): Note(s): ...[more].
Evidence: [HIFS] Human inference of function from sequence
[IMP] Inferred from mutant phenotype
Reference(s): [1] Stevenson G., et al., 1996
External database links:  
CAZY:
GT4
DIP:
DIP-11127N
ECOCYC:
G7095-MONOMER
ECOLIWIKI:
b2044
INTERPRO:
IPR001296
INTERPRO:
IPR028098
INTERPRO:
IPR023884
MINT:
MINT-1263848
MODBASE:
P71243
PFAM:
PF13439
PFAM:
PF00534
PRIDE:
P71243
REFSEQ:
NP_416548
SMR:
P71243
UNIPROT:
P71243


Operon      
Name: wcaKLM         
Operon arrangement:
Transcription unit        Promoter
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter wcaMp1 2115934 reverse Similarity to the consensus
Read more >
[ICWHO] [2]


Evidence    

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Stevenson G., Andrianopoulos K., Hobbs M., Reeves PR., 1996, Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid., J Bacteriol 178(16):4885-93

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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