RegulonDB RegulonDB 11.1: Gene Form
   

wzxC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

wzxC wcaJ wcaK wcaL wzxCp5 wzxCp5

Gene      
Name: wzxC    Texpresso search in the literature
Synonym(s): ECK2040, G7097, b2046, wzx
Genome position(nucleotides): 2118680 <-- 2120158
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.09
External database links:  
ASAP:
 ABE-0006783
ECHOBASE:
 EB3346
ECOLIHUB:
 wzxC
OU-MICROARRAY:
 b2046
STRING:
 511145.b2046
COLOMBOS: wzxC


Product      
Name: colanic acid repeat unit flippase
Synonym(s): Wzx, WzxC, colanic acid flippase
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 53.693
Isoelectric point: 10.306
Motif(s):
 
Type Positions Sequence Comment
13 -> 33 WSAIATVIIIGLGLVQMTVLA UniProt: Helical.
31 -> 325 VLARIIDNHQFGLLTVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLIGDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTCTVVSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTADSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQDDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIP
33 -> 33 A A → V: loss of substrate specificity; overcomes the lethality of MurJ deletion; retains the ability to support colanic acid synthesis and is able to support O-antigen synthesis (in a strain engineered to produce O-antigen polymers)
41 -> 41 F F → L: loss of substrate specificity; supports growth of a MurJ depletion strain; retains the ability to support colanic acid synthesis and is able to support O-antigen synthesis (in a strain engineered to produce O-antigen polymers)
43 -> 63 LLTVSLVIIALADTLSDFGIA UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.9.A - Transporters of Unknown Classification
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
biological_process GO:0009103 - lipopolysaccharide biosynthetic process
GO:0009242 - colanic acid biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Reeves PR., et al., 1996
[2] Reid AJ., et al., 2021
[3] Trisler P., et al., 1984
External database links:  
ALPHAFOLD:
 P77377
DIP:
 DIP-11136N
ECOCYC:
 G7097-MONOMER
ECOLIWIKI:
 b2046
PFAM:
 PF13440
PRIDE:
 P77377
PRODB:
 PRO_000024224
REFSEQ:
 NP_416550
UNIPROT:
 P77377


Operon      
Name: wcaCDEF-gmd-fcl-gmm-wcaI-cpsBG-wcaJ-wzxC         
Operon arrangement:
Transcription unit        Promoter
wcaCDEF-gmd-fcl-gmm-wcaI-cpsBG-wcaJ-wzxC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter wzxCp5 2120337 reverse nd [COMP-AINF] [4]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Reeves PR., Hobbs M., Valvano MA., Skurnik M., Whitfield C., Coplin D., Kido N., Klena J., Maskell D., Raetz CR., Rick PD., 1996, Bacterial polysaccharide synthesis and gene nomenclature., Trends Microbiol 4(12):495-503

 [2] Reid AJ., Eade CR., Jones KJ., Jorgenson MA., Troutman JM., 2021, Tracking Colanic Acid Repeat Unit Formation from Stepwise Biosynthesis Inactivation in Escherichia coli., Biochemistry 60(27):2221-2230

 [3] Trisler P., Gottesman S., 1984, lon transcriptional regulation of genes necessary for capsular polysaccharide synthesis in Escherichia coli K-12., J Bacteriol 160(1):184-91

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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