RegulonDB RegulonDB 10.8: Gene Form
   

wcaJ gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

wzxC wcaJ cpsG terminator wzxCp5 wzxCp5

Gene      
Name: wcaJ    Texpresso search in the literature
Synonym(s): ECK2041, G7098, b2047
Genome position(nucleotides): 2120160 <-- 2121554 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.62
External database links:  
ASAP:
ABE-0006785
ECHOBASE:
EB3345
OU-MICROARRAY:
b2047
PortEco:
wcaJ
STRING:
511145.b2047
COLOMBOS: wcaJ


Product      
Name: UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferase
Synonym(s): WcaJ, undecaprenyl-phosphate glucose phosphotransferase
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 52.408
Isoelectric point: 9.721
Motif(s):
 
Type Positions Sequence
16 -> 36 LISMVQRFSDITIMFAGLWLV
39 -> 59 VSGLSFLYMHLLVALITLVVF
105 -> 125 FDTQLKIWLAWYALTSIGLVV
312 -> 312 Q
279 -> 299 IVLATLILLLISPVLCCIALA

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.2 - colanic acid (M antigen)
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0016757 - transferase activity, transferring glycosyl groups
GO:0016740 - transferase activity
GO:0016780 - phosphotransferase activity, for other substituted phosphate groups
GO:0089702 - undecaprenyl-phosphate glucose phosphotransferase activity
biological_process GO:0000271 - polysaccharide biosynthetic process
GO:0009103 - lipopolysaccharide biosynthetic process
GO:0009242 - colanic acid biosynthetic process
Note(s): Note(s): ...[more].
Evidence: [HIFS] Human inference of function from sequence
[IMP] Inferred from mutant phenotype
Reference(s): [1] Hersch SJ., et al., 2020
[2] Patel KB., et al., 2012
[3] Ranjit DK., et al., 2016
[4] Stevenson G., et al., 1996
External database links:  
DIP:
DIP-11125N
ECOCYC:
G7098-MONOMER
ECOLIWIKI:
b2047
INTERPRO:
IPR003362
INTERPRO:
IPR017475
INTERPRO:
IPR017473
PFAM:
PF02397
PRIDE:
P71241
REFSEQ:
NP_416551
SMR:
P71241
UNIPROT:
P71241


Operon      
Name: wcaCDEF-gmd-fcl-gmm-wcaI-cpsBG-wcaJ-wzxC         
Operon arrangement:
Transcription unit        Promoter
wcaCDEF-gmd-fcl-gmm-wcaI-cpsBG-wcaJ-wzxC


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter wzxCp5 2120337 reverse Similarity to the consensus
Read more >
[ICWHO] [5]


Evidence    

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Hersch SJ., Watanabe N., Stietz MS., Manera K., Kamal F., Burkinshaw B., Lam L., Pun A., Li M., Savchenko A., Dong TG., 2020, Envelope stress responses defend against type six secretion system attacks independently of immunity proteins., Nat Microbiol

 [2] Patel KB., Toh E., Fernandez XB., Hanuszkiewicz A., Hardy GG., Brun YV., Bernards MA., Valvano MA., 2012, Functional characterization of UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferases of Escherichia coli and Caulobacter crescentus., J Bacteriol 194(10):2646-57

 [3] Ranjit DK., Young KD., 2016, Colanic Acid Intermediates Prevent De Novo Shape Recovery of Escherichia coli Spheroplasts, Calling into Question Biological Roles Previously Attributed to Colanic Acid., J Bacteriol 198(8):1230-40

 [4] Stevenson G., Andrianopoulos K., Hobbs M., Reeves PR., 1996, Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid., J Bacteriol 178(16):4885-93

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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