RegulonDB RegulonDB 11.1: Gene Form
   

hyfG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hyfG hyfF hyfE hyfH hyfI

Gene      
Name: hyfG    Texpresso search in the literature
Synonym(s): ECK2483, G7304, b2487
Genome position(nucleotides): 2608487 --> 2610154
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.32
Reference(s): [1] Andrews SC., et al., 1997
External database links:  
ASAP:
 ABE-0008194
ECHOBASE:
 EB3967
ECOLIHUB:
 hyfG
OU-MICROARRAY:
 b2487
STRING:
 511145.b2487
COLOMBOS: hyfG


Product      
Name: hydrogenase 4 catalytic subunit HyfG
Synonym(s): HyfG, hydrogenase 4, large subunit
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,membrane
Molecular weight: 63.383
Isoelectric point: 6.173
Motif(s):
 
Type Positions Sequence Comment
40 -> 155 VKINLLPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDA
220 -> 288 RGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLL
297 -> 447 VGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEG
445 -> 523 TEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDR

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0016020 - membrane
GO:0009326 - formate dehydrogenase complex
GO:0044569 - [Ni-Fe] hydrogenase complex
molecular_function GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0016651 - oxidoreductase activity, acting on NAD(P)H
GO:0016151 - nickel cation binding
GO:0051536 - iron-sulfur cluster binding
GO:0051540 - metal cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0008137 - NADH dehydrogenase (ubiquinone) activity
GO:0048038 - quinone binding
GO:0051287 - NAD binding
biological_process GO:0006007 - glucose catabolic process
GO:0009061 - anaerobic respiration
GO:0019645 - anaerobic electron transport chain
GO:0015944 - formate oxidation
Note(s): Note(s): ...[more].
Reference(s): [2] Trchounian K. 2012
External database links:  
ALPHAFOLD:
 P77329
DIP:
 DIP-9989N
ECOCYC:
 MONOMER0-150
ECOLIWIKI:
 b2487
INTERPRO:
 IPR014029
INTERPRO:
 IPR001268
INTERPRO:
 IPR037232
INTERPRO:
 IPR038290
INTERPRO:
 IPR001135
INTERPRO:
 IPR029014
INTERPRO:
 IPR001501
MODBASE:
 P77329
PFAM:
 PF00374
PFAM:
 PF00329
PFAM:
 PF00346
PRIDE:
 P77329
PRODB:
 PRO_000022969
PROSITE:
 PS00535
REFSEQ:
 NP_416982
SMR:
 P77329
UNIPROT:
 P77329


Operon      
Name: hyfABCDEFGHIJR-focB         
Operon arrangement:
Transcription unit        Promoter
hyfABCDEFGHIJR-focB


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, FhlA, HyfR
Repressed by: FNR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Andrews SC., Berks BC., McClay J., Ambler A., Quail MA., Golby P., Guest JR., 1997, A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system., Microbiology 143 ( Pt 11):3633-47

 [2] Trchounian K., 2012, Transcriptional control of hydrogen production during mixed carbon fermentation by hydrogenases 4 (hyf) and 3 (hyc) in Escherichia coli., Gene 506(1):156-60

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