RegulonDB

Gene
Name:	yfgG
Synonym(s):	ECK2500, G7315, b2504
Genome position(nucleotides):	2629290 --> 2629481 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	49.48
External database links:

ASAP:	ABE-0008247
ECHOBASE:	EB3955
OU-MICROARRAY:	b2504
PortEco:	yfgG
STRING:	511145.b2504
COLOMBOS:	yfgG

Product

Name:

nickel/cobalt stress response protein YfgG

Synonym(s):

YfgG

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

7.462

Isoelectric point:

12.398

Motif(s):

Type	Positions	Sequence
	9 -> 62	KRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHHQSKKEAQQSTLSVESPVQ
	20 -> 40	IVLLISFIFFFGRFIYSSVGA

Classification:

Multifun Terms (GenProtEC)
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	biological_process	GO:1990532 - stress response to nickel ion GO:0097501 - stress response to metal ion

Note(s):

Note(s): ...[more].

Reference(s):

[1] Price MN., et al., 2018

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

PRODOM:

REFSEQ:

UNIPROT:

Operon

Name:

yfgG

Operon arrangement:

Transcription unit	Promoter
yfgG	yfgGp

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yfgGp7	2629085	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 6.045. P-value: 3.581e-6 Read more >	[ICWHO]	[2]
promoter	yfgGp6	2629186	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.89. P-value: 1.605e-5 Read more >	[ICWHO]	[2]
promoter	yfgGp5	2629252	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.85. P-value: 6.507e-5 Read more >	[ICWHO], [RS-EPT-CBR]	[2], [3]
promoter	yfgHp10	2629672	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.28. P-value: 5.328e-5 Read more >	[ICWHO]	[2]
promoter	TSS_2784	2629763	forward	nd	[RS-EPT-CBR]	[3]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Price MN., Wetmore KM., Waters RJ., Callaghan M., Ray J., Liu H., Kuehl JV., Melnyk RA., Lamson JS., Suh Y., Carlson HK., Esquivel Z., Sadeeshkumar H., Chakraborty R., Zane GM., Rubin BE., Wall JD., Visel A., Bristow J., Blow MJ., Arkin AP., Deutschbauer AM., 2018, Mutant phenotypes for thousands of bacterial genes of unknown function., Nature 557(7706):503-509
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.