RegulonDB RegulonDB 11.0: Gene Form
   

tapT gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

patZ tapT trxC CRP CRP terminator pssAp4 pssAp4 TSS_2894 TSS_2894 TSS_2893 TSS_2893 pkap pkap TSS_2892 TSS_2892

Gene      
Name: tapT    Texpresso search in the literature
Synonym(s): ECK2581, G7349, b2583, tuaA, yfiP
Genome position(nucleotides): 2719223 --> 2719921
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.36
External database links:  
ASAP:
ABE-0008503
ECHOBASE:
EB3975
ECOLIHUB:
yfiP
OU-MICROARRAY:
b2583
STRING:
511145.b2583
COLOMBOS: tapT


Product      
Name: tRNA 3-amino-3-carboxypropyltransferase
Synonym(s): DTW domain-containing protein YfiP, TapT, TuaA, YfiP, tRNA aminocarboxypropyltransferase
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 25.995
Isoelectric point: 8.112
Motif(s):
 
Type Positions Sequence Comment
1 -> 20 MTENAVLQLRAERIARATRP UniProt: Complete loss of activity..
30 -> 210 RCQRCLLPEKLCLCSTITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNPDYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVISVDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFK
31 -> 31 C UniProt: Complete loss of activity; when associated with S-34..
34 -> 34 C UniProt: Complete loss of activity; when associated with S-31..
41 -> 41 C UniProt: Shows weak residual activity; when associated with S-43..

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016432 - tRNA-uridine aminocarboxypropyltransferase activity
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0008270 - zinc ion binding
biological_process GO:0006400 - tRNA modification
GO:0008033 - tRNA processing
Note(s): Note(s): ...[more].
Reference(s): [1] Burroughs AM., et al., 2014
[2] Ohashi Z., et al., 1974
External database links:  
ALPHAFOLD:
Q47319
DIP:
DIP-12067N
ECOCYC:
G7349-MONOMER
ECOLIWIKI:
b2583
INTERPRO:
IPR005636
INTERPRO:
IPR039262
PANTHER:
PTHR21392
PFAM:
PF03942
PRIDE:
Q47319
REFSEQ:
NP_417078
SMART:
SM01144
UNIPROT:
Q47319


Operon      
Name: tapT-patZ         
Operon arrangement:
Transcription unit        Promoter
patZ
tapT-patZ


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2892 2718965 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2893 2722287 forward nd [RS-EPT-CBR] [3]
  promoter TSS_2894 2722503 forward nd [RS-EPT-CBR] [3]
  promoter pssAp4 2722578 forward nd [ICWHO] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Burroughs AM., Aravind L., 2014, Analysis of two domains with novel RNA-processing activities throws light on the complex evolution of ribosomal RNA biogenesis., Front Genet 5:424

 [2] Ohashi Z., Maeda M., McCloskey JA., Nishimura S., 1974, 3-(3-Amino-3-carboxypropyl)uridine: a novel modified nucleoside isolated from Escherichia coli phenylalanine transfer ribonucleic acid., Biochemistry 13(12):2620-5

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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