RegulonDB

Gene
Name:	mltB
Synonym(s):	ECK2696, G7410, b2701, slt
Genome position(nucleotides):	2824491 <-- 2825576
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	55.16
External database links:

ASAP:	ABE-0008885
ECHOBASE:	EB2561
ECOLIHUB:	mltB
OU-MICROARRAY:	b2701
STRING:	511145.b2701
COLOMBOS:	mltB

Gene

Name:

mltB

Synonym(s):

ECK2696, G7410, b2701, slt

Genome position(nucleotides):

2824491 <-- 2825576

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

55.16

External database links:

ASAP:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Type

Positions

Sequence

Comment

1 -> 18

MFKRRYVTLLPLFVLLAA

19 -> 39

CSSKPKPTETDTTTGTPSGGF

40 -> 98

LLEPQHNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQ

57 -> 353

NPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTTSVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIGVETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFAGAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMANGQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGQ

109 -> 169

GPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIGVETRWGRVM

Type

Positions

Sequence

Comment

1 -> 18

MFKRRYVTLLPLFVLLAA

19 -> 39

CSSKPKPTETDTTTGTPSGGF

40 -> 98

LLEPQHNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQ

57 -> 353

109 -> 169

GPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIGVETRWGRVM

162 -> 162

E → Q: loss of function mutation

170 -> 215

GKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKG

216 -> 269

SFAGAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGD

237 -> 251

DFSGDGHINLWDPVD

contains 6 ligating residues (Asp237, Ser239, Asp241, His243 and Asp251)

270 -> 329

QVAVMANGQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGL

330 -> 361

PNFYTITRYNHSTHYAMAVWQLGQAVALARVQ

Multifun Terms (GenProtEC)

1 - metabolism --> 1.2 - degradation of macromolecules --> 1.2.3 - proteins/peptides/glycopeptides

Gene Ontology Terms (GO)

cellular_component

GO:0009279 - cell outer membrane
GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0036405 - anchored component of cell outer membrane

molecular_function

GO:0005515 - protein binding
GO:0016829 - lyase activity
GO:0008933 - lytic transglycosylase activity
GO:0005509 - calcium ion binding
GO:0031402 - sodium ion binding
GO:0008932 - lytic endotransglycosylase activity

biological_process

GO:0009253 - peptidoglycan catabolic process
GO:0071555 - cell wall organization

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_3016	2825541		reverse	nd	[RS-EPT-CBR]	[16]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

TSS_3016

2825541

reverse

[RS-EPT-CBR]

[16]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Evidence

[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Blackburn NT., Clarke AJ., 2001, Identification of four families of peptidoglycan lytic transglycosylases., J Mol Evol 52(1):78-84
[2] Brokx SJ., Ellison M., Locke T., Bottorff D., Frost L., Weiner JH., 2004, Genome-wide analysis of lipoprotein expression in Escherichia coli MG1655., J Bacteriol 186(10):3254-8
[3] Gill RT., DeLisa MP., Valdes JJ., Bentley WE., 2001, Genomic analysis of high-cell-density recombinant Escherichia coli fermentation and "cell conditioning" for improved recombinant protein yield., Biotechnol Bioeng 72(1):85-95
[4] Hamdallah I., Torok N., Bischof KM., Majdalani N., Chadalavada S., Mdluli N., Creamer KE., Clark M., Holdener C., Basting PJ., Gottesman S., Slonczewski JL., 2018, Experimental Evolution of Escherichia coli K-12 at High pH and with RpoS Induction., Appl Environ Microbiol 84(15)
[5] Heidrich C., Ursinus A., Berger J., Schwarz H., Holtje JV., 2002, Effects of multiple deletions of murein hydrolases on viability, septum cleavage, and sensitivity to large toxic molecules in Escherichia coli., J Bacteriol 184(22):6093-9
[6] Korsak D., Liebscher S., Vollmer W., 2005, Susceptibility to antibiotics and beta-lactamase induction in murein hydrolase mutants of Escherichia coli., Antimicrob Agents Chemother 49(4):1404-9
[7] Maurer LM., Yohannes E., Bondurant SS., Radmacher M., Slonczewski JL., 2005, pH regulates genes for flagellar motility, catabolism, and oxidative stress in Escherichia coli K-12., J Bacteriol 187(1):304-19
[8] Meisel U., Holtje JV., Vollmer W., 2003, Overproduction of inactive variants of the murein synthase PBP1B causes lysis in Escherichia coli., J Bacteriol 185(18):5342-8
[9] Molloy MP., Herbert BR., Slade MB., Rabilloud T., Nouwens AS., Williams KL., Gooley AA., 2000, Proteomic analysis of the Escherichia coli outer membrane., Eur J Biochem 267(10):2871-81
[10] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3
[11] Romeis T., Vollmer W., Holtje JV., 1993, Characterization of three different lytic transglycosylases in Escherichia coli., FEMS Microbiol Lett 111(2-3):141-6
[12] Schiffer G., Holtje JV., 1999, Cloning and characterization of PBP 1C, a third member of the multimodular class A penicillin-binding proteins of Escherichia coli., J Biol Chem 274(45):32031-9
[13] Van Dyk TK., DeRose EJ., Gonye GE., 2001, LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains., J Bacteriol 183(19):5496-505
[14] Vijayakumar SR., Kirchhof MG., Patten CL., Schellhorn HE., 2004, RpoS-regulated genes of Escherichia coli identified by random lacZ fusion mutagenesis., J Bacteriol 186(24):8499-507
[15] Vollmer W., von Rechenberg M., Holtje JV., 1999, Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli., J Biol Chem 274(10):6726-34
[16] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Blackburn NT., Clarke AJ., 2001, Identification of four families of peptidoglycan lytic transglycosylases., J Mol Evol 52(1):78-84

[2] Brokx SJ., Ellison M., Locke T., Bottorff D., Frost L., Weiner JH., 2004, Genome-wide analysis of lipoprotein expression in Escherichia coli MG1655., J Bacteriol 186(10):3254-8

[3] Gill RT., DeLisa MP., Valdes JJ., Bentley WE., 2001, Genomic analysis of high-cell-density recombinant Escherichia coli fermentation and "cell conditioning" for improved recombinant protein yield., Biotechnol Bioeng 72(1):85-95

[4] Hamdallah I., Torok N., Bischof KM., Majdalani N., Chadalavada S., Mdluli N., Creamer KE., Clark M., Holdener C., Basting PJ., Gottesman S., Slonczewski JL., 2018, Experimental Evolution of Escherichia coli K-12 at High pH and with RpoS Induction., Appl Environ Microbiol 84(15)

[5] Heidrich C., Ursinus A., Berger J., Schwarz H., Holtje JV., 2002, Effects of multiple deletions of murein hydrolases on viability, septum cleavage, and sensitivity to large toxic molecules in Escherichia coli., J Bacteriol 184(22):6093-9

[6] Korsak D., Liebscher S., Vollmer W., 2005, Susceptibility to antibiotics and beta-lactamase induction in murein hydrolase mutants of Escherichia coli., Antimicrob Agents Chemother 49(4):1404-9

[7] Maurer LM., Yohannes E., Bondurant SS., Radmacher M., Slonczewski JL., 2005, pH regulates genes for flagellar motility, catabolism, and oxidative stress in Escherichia coli K-12., J Bacteriol 187(1):304-19

[8] Meisel U., Holtje JV., Vollmer W., 2003, Overproduction of inactive variants of the murein synthase PBP1B causes lysis in Escherichia coli., J Bacteriol 185(18):5342-8

[9] Molloy MP., Herbert BR., Slade MB., Rabilloud T., Nouwens AS., Williams KL., Gooley AA., 2000, Proteomic analysis of the Escherichia coli outer membrane., Eur J Biochem 267(10):2871-81

[10] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3

[11] Romeis T., Vollmer W., Holtje JV., 1993, Characterization of three different lytic transglycosylases in Escherichia coli., FEMS Microbiol Lett 111(2-3):141-6

[12] Schiffer G., Holtje JV., 1999, Cloning and characterization of PBP 1C, a third member of the multimodular class A penicillin-binding proteins of Escherichia coli., J Biol Chem 274(45):32031-9

[13] Van Dyk TK., DeRose EJ., Gonye GE., 2001, LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains., J Bacteriol 183(19):5496-505

[14] Vijayakumar SR., Kirchhof MG., Patten CL., Schellhorn HE., 2004, RpoS-regulated genes of Escherichia coli identified by random lacZ fusion mutagenesis., J Bacteriol 186(24):8499-507

[15] Vollmer W., von Rechenberg M., Holtje JV., 1999, Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli., J Biol Chem 274(10):6726-34

[16] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.