RegulonDB RegulonDB 11.0: Gene Form
   

ispD gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

truD ispD ispF ftsB umpG ispFp3 ispFp3 ispFp2 ispFp2 surEp2 surEp2

Gene      
Name: ispD    Texpresso search in the literature
Synonym(s): ECK2742, G7423, b2747, ygbP
Genome position(nucleotides): 2871780 <-- 2872490
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.41
External database links:  
ASAP:
 ABE-0009017
ECHOBASE:
 EB2913
ECOLIHUB:
 ispD
OU-MICROARRAY:
 b2747
STRING:
 511145.b2747
COLOMBOS: ispD


Product      
Name: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Synonym(s): 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, IspD, YgbP
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 25.737
Isoelectric point: 6.649
Motif(s):
 
Type Positions Sequence Comment
2 -> 236 ATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHALLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWVLVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGLWHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPEDLALAEFYLTRTIHQENT UniProt: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase.
8 -> 229 VCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHALLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWVLVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGLWHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPEDLALAEFYLTR
27 -> 27 K UniProt: Strong decrease in activity..
140 -> 140 T UniProt: Loss of activity..
191 -> 191 E UniProt: Loss of activity..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.11 - menaquinone, ubiquinone
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016779 - nucleotidyltransferase activity
GO:0016740 - transferase activity
GO:0050518 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
GO:0000287 - magnesium ion binding
GO:0042802 - identical protein binding
GO:0070567 - cytidylyltransferase activity
biological_process GO:0008299 - isoprenoid biosynthetic process
GO:0016114 - terpenoid biosynthetic process
GO:0019288 - isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
External database links:  
ALPHAFOLD:
 Q46893
ECOCYC:
 G7423-MONOMER
ECOLIWIKI:
 b2747
INTERPRO:
 IPR029044
INTERPRO:
 IPR034683
INTERPRO:
 IPR018294
INTERPRO:
 IPR001228
MODBASE:
 Q46893
PDB:
 1I52
PDB:
 1INI
PDB:
 1INJ
PDB:
 1VGT
PDB:
 1VGU
PDB:
 3N9W
PDB:
 1H3M
PFAM:
 PF01128
PRIDE:
 Q46893
PRODB:
 PRO_000023036
PROSITE:
 PS01295
REFSEQ:
 NP_417227
SMR:
 Q46893
UNIPROT:
 Q46893


Operon      
Name: ispDF         
Operon arrangement:
Transcription unit        Promoter
ispDF


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ispFp2 2871911 reverse nd [ICWHO] [1]
  promoter ispFp3 2871984 reverse nd [ICWHO] [1]


Evidence    

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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