RegulonDB

Gene
Name:	casE
Synonym(s):	ECK2751, G7426, b2756, cas6e, cse3, ygcH
Genome position(nucleotides):	2879788 <-- 2880387 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	45.33
External database links:

ASAP:	ABE-0009042
ECHOBASE:	EB2916
OU-MICROARRAY:	b2756
PortEco:	casE
STRING:	511145.b2756
COLOMBOS:	casE

Product

Name:

pre-CRISPR RNA endonuclease

Synonym(s):

Cas6e, CasE, YgcH, crRNA endonuclease

Sequence:

Get amino acid sequence Fasta Format

Molecular weight:

22.293

Isoelectric point:

10.087

Motif(s):

Type	Positions	Sequence
	1 -> 198	MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKRNTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKRLDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQTVCFEGVLTINDAPALIDLVQQGIGPAKSMGCGLLSLAP
	20 -> 20	H
	2 -> 199	YLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKRNTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKRLDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQTVCFEGVLTINDAPALIDLVQQGIGPAKSMGCGLLSLAPL

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		5 - cell processes --> 5.10 - defense/survival
Gene Ontology Terms (GO)
	cellular_component	GO:0032991 - protein-containing complex
	molecular_function	GO:0004518 - nuclease activity GO:0004519 - endonuclease activity GO:0005515 - protein binding GO:0016787 - hydrolase activity GO:0003723 - RNA binding GO:0004521 - endoribonuclease activity
	biological_process	GO:0006396 - RNA processing GO:0051607 - defense response to virus GO:0090502 - RNA phosphodiester bond hydrolysis, endonucleolytic

Note(s):

Note(s): ...[more].

Reference(s):

[1] Datsenko KA., et al., 2012
[2] Semenova E., et al., 2015
[3] van Duijn E., et al., 2012

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Operon

Name:

casABCDE12

Operon arrangement:

Transcription unit	Promoter
ygbF	ygbFp
casABCDE12	casAp
casDE-ygbTF	casDp

Transcriptional Regulation
	Display Regulation
Activated by:	SlyA, LeuO
Repressed by:	CRP, H-NS

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Datsenko KA., Pougach K., Tikhonov A., Wanner BL., Severinov K., Semenova E., 2012, Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system., Nat Commun 3:945
[2] Semenova E., Kuznedelov K., Datsenko KA., Boudry PM., Savitskaya EE., Medvedeva S., Beloglazova N., Logacheva M., Yakunin AF., Severinov K., 2015, The Cas6e ribonuclease is not required for interference and adaptation by the E. coli type I-E CRISPR-Cas system., Nucleic Acids Res 43(12):6049-61
[3] van Duijn E., Barbu IM., Barendregt A., Jore MM., Wiedenheft B., Lundgren M., Westra ER., Brouns SJ., Doudna JA., van der Oost J., Heck AJ., 2012, Native tandem and ion mobility mass spectrometry highlight structural and modular similarities in clustered-regularly-interspaced shot-palindromic-repeats (CRISPR)-associated protein complexes from Escherichia coli and Pseudomonas aeruginosa., Mol Cell Proteomics 11(11):1430-41