RegulonDB RegulonDB 11.0: Gene Form
   

casA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cas3 casA casC casB LeuO CRP LeuO StpA H-NS LeuO LeuO SlyA TSS_3043 TSS_3043 casAp casAp

Gene      
Name: casA    Texpresso search in the literature
Synonym(s): ECK2755, G7430, b2760, cse1, ygcL
Genome position(nucleotides): 2882630 <-- 2884138
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
43.8
External database links:  
ASAP:
ABE-0009050
ECHOBASE:
EB2920
ECOLIHUB:
casA
OU-MICROARRAY:
b2760
STRING:
511145.b2760
COLOMBOS: casA


Product      
Name: type I-E CRISPR system Cascade subunit CasA
Synonym(s): CasA, Cascade subunit A, Cse1, YgcL
Sequence: Get amino acid sequence Fasta Format
Molecular weight: 55.901
Isoelectric point: 8.346
Motif(s):
 
Type Positions Sequence Comment
2 -> 459 NLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDMELAALALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQTKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGGGFKSGLRGGTPVTTFVRGIDLRSTVLLNVLTLPRLQKQFPNESHTENQPTWIKPIKSNESIPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGLWPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQFRNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTALRKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANVNFSQADEVIADLRDKLHQLCE
129 -> 129 F UniProt: 80% increase in phage sensitivity; 500-fold decrease in affinity for target dsDNA..
130 -> 130 V UniProt: 20% increase in phage sensitivity; no change in binding of target dsDNA..
131 -> 131 N UniProt: 45% increase in phage sensitivity; 60-fold decrease in affinity for target dsDNA..
288 -> 294 VKKGEVE loop region implicated in Cas3 helicase-nuclease binding

 

Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.10 - defense/survival
Gene Ontology Terms (GO)  
cellular_component GO:0032991 - protein-containing complex
molecular_function GO:0003677 - DNA binding
GO:0005515 - protein binding
GO:0003723 - RNA binding
GO:0008270 - zinc ion binding
biological_process GO:0099048 - CRISPR-cas system
GO:0051607 - defense response to virus
Note(s): Note(s): ...[more].
Reference(s): [1] Hochstrasser ML., et al., 2014
[2] Sashital DG., et al., 2012
External database links:  
ALPHAFOLD:
Q46901
DIP:
DIP-12128N
ECOCYC:
G7430-MONOMER
ECOLIWIKI:
b2760
INTERPRO:
IPR013381
PDB:
5CD4
PDB:
5H9F
PDB:
5H9E
PDB:
4U7U
PDB:
4TVX
PDB:
4QYZ
PFAM:
PF09481
PRIDE:
Q46901
REFSEQ:
NP_417240
SMR:
Q46901
UNIPROT:
Q46901


Operon      
Name: casABCDE12         
Operon arrangement:
Transcription unit        Promoter
ygbF
casABCDE12
casDE-ygbTF


Transcriptional Regulation      
Display Regulation             
Activated by: SlyA, LeuO, StpA
Repressed by: CRP, H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3043 2884659 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Hochstrasser ML., Taylor DW., Bhat P., Guegler CK., Sternberg SH., Nogales E., Doudna JA., 2014, CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference., Proc Natl Acad Sci U S A 111(18):6618-23

 [2] Sashital DG., Wiedenheft B., Doudna JA., 2012, Mechanism of foreign DNA selection in a bacterial adaptive immune system., Mol Cell 46(5):606-15

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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