RegulonDB

Gene
Name:	aaeR
Synonym(s):	ECK3232, G7688, b3243, qseA, yhcS
Genome position(nucleotides):	3389520 --> 3390449
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.9
External database links:

ASAP:	ABE-0010639
ECHOBASE:	EB2676
ECOLIHUB:	aaeR
OU-MICROARRAY:	b3243
STRING:	511145.b3243
COLOMBOS:	aaeR

Product

Name:

DNA-binding transcriptional activator AaeR

Synonym(s):

AaeR, LysR-type transcriptional regulator AaeR, QseA, YhcS, quorum sensing transcriptional regulator of LYSR-type

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

34.516

Isoelectric point:

8.419

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 59	MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLT	UniProt: HTH lysR-type.
	4 -> 63	LKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDELQVKLLNRSTRSIGLTEAGR
	19 -> 38	FTAAARQLQMSVSSISQTVS	UniProt: H-T-H motif.
	90 -> 290	PIGTLRIGCSSTMAQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGAMPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINSLTD

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
		5 - cell processes --> 5.10 - defense/survival
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0003700 - DNA-binding transcription factor activity GO:0000976 - transcription cis-regulatory region binding
	biological_process	GO:0006351 - transcription, DNA-templated GO:0006355 - regulation of transcription, DNA-templated GO:0006352 - DNA-templated transcription, initiation GO:0045893 - positive regulation of transcription, DNA-templated

Note(s):

Note(s): ...[more].

Evidence:

[COMP-HINF] Inferred by a human based on computational evidence
[COMP-HINF-FN-FROM-SEQ] Human inference of function from sequence
[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[1] Perez-Rueda E., et al., 2000
[2] Perez-Rueda E., et al., 2004
[3] Sperandio V., et al., 2002
[4] Van Dyk TK., et al., 2004

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Repressed by:	CRP

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	aaeRp10	3389381	forward	nd	[COMP-AINF]	[5]
promoter	aaeRp9	3389383	forward	nd	[COMP-AINF]	[5]
promoter	aaeRp7	3389461	forward	nd	[COMP-AINF]	[5]
promoter	aaeRp6	3389477	forward	nd	[COMP-AINF]	[5]

Reference(s)
[1] Perez-Rueda E., Collado-Vides J., 2000, The repertoire of DNA-binding transcriptional regulators in Escherichia coli K-12., Nucleic Acids Res 28(8):1838-47
[2] Perez-Rueda E., Collado-Vides J., Segovia L., 2004, Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea., Comput Biol Chem 28(5-6):341-50
[3] Sperandio V., Li CC., Kaper JB., 2002, Quorum-sensing Escherichia coli regulator A: a regulator of the LysR family involved in the regulation of the locus of enterocyte effacement pathogenicity island in enterohemorrhagic E. coli., Infect Immun 70(6):3085-93
[4] Van Dyk TK., Templeton LJ., Cantera KA., Sharpe PL., Sariaslani FS., 2004, Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?, J Bacteriol 186(21):7196-204
[5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78