RegulonDB RegulonDB 10.9: Gene Form
   

wecF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

wzyE wzxE wecE wecF rffM TSS_4448 TSS_4448 TSS_4447 (cluster) TSS_4447 (cluster) TSS_4446 TSS_4446 TSS_4445 (cluster) TSS_4445 (cluster)

Gene      
Name: wecF    Texpresso search in the literature
Synonym(s): ECK3785, G7800, b4404, b4405, b4481, rff, rffT, yifM
Genome position(nucleotides): 3977525 --> 3978604 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.87
External database links:  
ASAP:
ABE-0174115
ECHOBASE:
EB4066
ECOLIHUB:
rffT
OU-MICROARRAY:
b4404
STRING:
511145.b4481
COLOMBOS: wecF


Product      
Name: TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase
Synonym(s): Rff, RffT, WecF, YifM
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 40.64
Isoelectric point: 7.614
Motif(s):
 
Type Positions Sequence
132 -> 132 A
1 -> 355 MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGELLFFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKNTIAFFSPNYLQGWQRALAIAA

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.4 - enterobacterial common antigen (surface glycolipid)
  6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
molecular_function GO:0016757 - transferase activity, transferring glycosyl groups
GO:0016758 - transferase activity, transferring hexosyl groups
GO:0016740 - transferase activity
GO:0008417 - fucosyltransferase activity
GO:0102031 - 4-acetamido-4,6-dideoxy-D-galactose transferase activity
biological_process GO:0009246 - enterobacterial common antigen biosynthetic process
GO:0036065 - fucosylation
Note(s): Note(s): ...[more].
Reference(s): [1] Meier-Dieter U., et al., 1992
[2] Ohta M., et al., 1991
[3] Schmidt G., et al., 1976
External database links:  
CAZY:
GT56
ECOCYC:
G7800-MONOMER
ECOLIWIKI:
b4481
INTERPRO:
IPR009993
PFAM:
PF07429
PRIDE:
P56258
PRODB:
PRO_000024220
REFSEQ:
YP_026257
UNIPROT:
P56258


Operon      
Name: rfe-wzzE-wecBC-rffGHC-wecE-wzxE-wecF-wzyE-rffM         
Operon arrangement:
Transcription unit        Promoter
rfe-wzzE-wecBC-rffGHC-wecE-wzxE-wecF-wzyE-rffM


Transcriptional Regulation      
Display Regulation             
Repressed by: NsrR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4445 (cluster) 3977392 forward For this promoter, there
Read more >
[RS-EPT-CBR] [4]
  promoter TSS_4446 3979755 forward nd [RS-EPT-CBR] [4]
  promoter TSS_4447 (cluster) 3979832 forward For this promoter, there
Read more >
[RS-EPT-CBR] [4]
  promoter TSS_4448 3979835 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Meier-Dieter U., Barr K., Starman R., Hatch L., Rick PD., 1992, Nucleotide sequence of the Escherichia coli rfe gene involved in the synthesis of enterobacterial common antigen. Molecular cloning of the rfe-rff gene cluster., J Biol Chem 267(2):746-53

 [2] Ohta M., Ina K., Kusuzaki K., Kido N., Arakawa Y., Kato N., 1991, Cloning and expression of the rfe-rff gene cluster of Escherichia coli., Mol Microbiol 5(8):1853-62

 [3] Schmidt G., Mayer H., Makela PH., 1976, Presence of rfe genes in Escherichia coli: their participation in biosynthesis of O antigen and enterobacterial common antigen., J Bacteriol 127(2):755-62

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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