RegulonDB

Gene
Name:	yjfN
Synonym(s):	ECK4184, G7851, b4188
Genome position(nucleotides):	4416017 <-- 4416292 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.09
External database links:

ASAP:	ABE-0013704
ECHOBASE:	EB2412
ECOLIHUB:	yjfN
OU-MICROARRAY:	b4188
STRING:	511145.b4188
COLOMBOS:	yjfN

Product

Name:

protease activator YjfN

Synonym(s):

YjfN

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

periplasmic space

Molecular weight:

9.949

Isoelectric point:

3.961

Motif(s):

Type	Positions	Sequence
	1 -> 21	MKQLLASPSLQLVTYPASATA
	91 -> 91	A
	22 -> 91	QSAEFASADCVTGLNEIGQISVSNISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA
	35 -> 90	LNEIGQISVSNISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILY
	83 -> 83	W

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.3 - inhibition / activation of enzymes
Gene Ontology Terms (GO)
	cellular_component	GO:0042597 - periplasmic space
	molecular_function	GO:0061133 - endopeptidase activator activity
	biological_process	GO:0010950 - positive regulation of endopeptidase activity

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Butland G., et al., 2005
[2] Chang DE., et al., 2002
[3] Nobre LS., et al., 2009
[4] Song I., et al., 2018

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

yjfN

Operon arrangement:

Transcription unit	Promoter
yjfN

RNA cis-regulatory element

Attenuation:	Translational

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_5053	4415541		forward	nd	[RS-EPT-CBR]	[5]
	promoter	yjfPp4	4416756		forward	nd	[ICWHO]	[6]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Butland G., Peregrin-Alvarez JM., Li J., Yang W., Yang X., Canadien V., Starostine A., Richards D., Beattie B., Krogan N., Davey M., Parkinson J., Greenblatt J., Emili A., 2005, Interaction network containing conserved and essential protein complexes in Escherichia coli., Nature 433(7025):531-7
[2] Chang DE., Smalley DJ., Conway T., 2002, Gene expression profiling of Escherichia coli growth transitions: an expanded stringent response model., Mol Microbiol 45(2):289-306
[3] Nobre LS., Al-Shahrour F., Dopazo J., Saraiva LM., 2009, Exploring the antimicrobial action of a carbon monoxide-releasing compound through whole-genome transcription profiling of Escherichia coli., Microbiology 155(Pt 3):813-24
[4] Song I., Eom G., Kim S., 2018, A Small Periplasmic Protein with a Hydrophobic C-Terminal Residue Enhances DegP Proteolysis as a Suicide Activator., J Bacteriol 200(3)
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78