RegulonDB RegulonDB 11.1: Gene Form
   

thiM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

yegX thiD thiM rcnR Cis-reg; riboswitch terminator terminator thiMp thiMp thiDp3 thiDp3 yegXp6 yegXp6 yegXp1 yegXp1

Gene      
Name: thiM    Texpresso search in the literature
Synonym(s): ECK2097, M007, b2104
Genome position(nucleotides): 2184513 <-- 2185301
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.12
External database links:  
ASAP:
 ABE-0006960
ECHOBASE:
 EB3822
ECOLIHUB:
 thiM
OU-MICROARRAY:
 b2104
STRING:
 511145.b2104
COLOMBOS: thiM


Product      
Name: hydroxyethylthiazole kinase
Synonym(s): ThiM
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 27.339
Isoelectric point: 5.919
Motif(s):
 
Type Positions Sequence Comment
15 -> 259 LHLFHQHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVAVGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTLARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTLENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQE

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.8 - thiamin
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0052673 - prenol kinase activity
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004417 - hydroxyethylthiazole kinase activity
GO:0000287 - magnesium ion binding
GO:0042802 - identical protein binding
biological_process GO:0009228 - thiamine biosynthetic process
GO:0016310 - phosphorylation
GO:0009229 - thiamine diphosphate biosynthetic process
GO:0036172 - thiamine salvage
External database links:  
ALPHAFOLD:
 P76423
ECOCYC:
 THZ-KIN-MONOMER
ECOLIWIKI:
 b2104
INTERPRO:
 IPR000417
INTERPRO:
 IPR029056
MODBASE:
 P76423
PFAM:
 PF02110
PRIDE:
 P76423
PRINTS:
 PR01099
PRODB:
 PRO_000024062
REFSEQ:
 NP_416607
SMR:
 P76423
SWISSMODEL:
 P76423
UNIPROT:
 P76423


Operon      
Name: thiMD         
Operon arrangement:
Transcription unit        Promoter
thiMD


RNA cis-regulatory element    
Attenuation: Translational
   
Cis-reg; riboswitch TPP riboswitch (THI element)
   
   


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yegXp1 2183801 reverse nd [COMP-AINF] [1]
  promoter yegXp6 2183820 reverse nd [COMP-AINF] [1]
  promoter thiDp3 2184673 reverse nd [COMP-AINF] [1]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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