RegulonDB RegulonDB 11.1: Gene Form
   

mhpC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mhpB mhpC mhpD mhpF

Gene      
Name: mhpC    Texpresso search in the literature
Synonym(s): ECK0346, M012, b0349
Genome position(nucleotides): 371239 --> 372105
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.67
External database links:  
ASAP:
 ABE-0001201
ECHOBASE:
 EB4168
ECOLIHUB:
 mhpC
OU-MICROARRAY:
 b0349
STRING:
 511145.b0349
COLOMBOS: mhpC


Product      
Name: 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase
Synonym(s): MhpC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 31.937
Isoelectric point: 6.722
Motif(s):
 
Type Positions Sequence Comment
2 -> 288 SYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGSGPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSVVNSGSRSDLNARILKSVVDQLDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLVLNFLARP UniProt: 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase.
38 -> 279 VVLLHGSGPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSVVNSGSRSDLNARILKSVVDQLDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQ
44 -> 44 S UniProt: 2-fold decrease in catalytic efficiency and more than 5-fold increase in affinity for the natural substrate..
113 -> 113 N UniProt: 200-fold decrease in catalytic activity and almost 2-fold increase in affinity..
114 -> 114 S UniProt: Weakly active. 3-fold decrease in affinity. Fast ketonisation and slow C-C cleavage..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016787 - hydrolase activity
GO:0042803 - protein homodimerization activity
GO:0018771 - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase activity
GO:0052823 - 2-hydroxy-6-oxonona-2,4,7-trienedioate hydrolase activity
biological_process GO:0019439 - aromatic compound catabolic process
GO:0019380 - 3-phenylpropionate catabolic process
GO:0019622 - 3-(3-hydroxy)phenylpropionate catabolic process
External database links:  
ALPHAFOLD:
 P77044
DIP:
 DIP-10207N
ECOCYC:
 MHPCHYDROL-MONOMER
ECOLIWIKI:
 b0349
INTERPRO:
 IPR023791
INTERPRO:
 IPR000639
INTERPRO:
 IPR000073
INTERPRO:
 IPR029058
MODBASE:
 P77044
PDB:
 1U2E
PFAM:
 PF12697
PRIDE:
 P77044
PRINTS:
 PR00111
PRINTS:
 PR00412
PRODB:
 PRO_000023230
REFSEQ:
 NP_414883
SMR:
 P77044
UNIPROT:
 P77044


Operon      
Name: mhpABCDFE         
Operon arrangement:
Transcription unit        Promoter
mhpABCDFE


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, MhpR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References



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