RegulonDB RegulonDB 11.1: Gene Form
   

insAB5 gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

insAB5 insB5 flhD insA5 OmpR LrhA IHF CRP AcrR YjjQ RcsAB FliZ OmpR Fur terminator TSS_2232 TSS_2232 flhDp2 flhDp2 flhDp flhDp flhCp3 flhCp3

Gene      
Name: insAB5    Texpresso search in the literature
Synonym(s): G0-10539
Genome position(nucleotides): 1978518 <-- 1979215
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.15
External database links:  
ECOLIHUB:
 insAB-5
COLOMBOS: insAB5


Product      
Name: InsAB' transposase
Synonym(s): InsAB-5
Sequence: Get amino acid sequence Fasta Format
Molecular weight: 26.42
Isoelectric point: 11.491
Motif(s):
 
Type Positions Sequence Comment
1 -> 36 MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCR
43 -> 86 FTYTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHLKN
102 -> 232 SSSARKWTNSGAMSGLNRASAGCFTRMTVSGRRLLRTYSVNALWRRWGVL*ACCHPLTW*YG*RMAGRCMNPA*RESCT*SASDIRSELSGIT*I*GSTWHGWDGSRCRSQNRWSCMTKSSGII*T*NTI

 
Classification:
Multifun Terms (GenProtEC)  
  8 - extrachromosomal --> 8.3 - transposon related
Gene Ontology Terms (GO)  
External database links:  
ECOCYC:
 MONOMER0-4229
PFAM:
 PF12759
PFAM:
 PF03400
PFAM:
 PF03811


Operon      
Name: insAB5A5B5         
Operon arrangement:
Transcription unit        Promoter
insA5B5AB5


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter flhCp3 1977932 reverse nd [COMP-AINF] [1]
  promoter TSS_2232 1979050 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2233 1979233 reverse nd [RS-EPT-CBR] [2]
  promoter uspCp8 1979586 forward nd [COMP-AINF] [1]
  promoter uspCp9 1979591 forward nd [COMP-AINF] [1]
  promoter uspCp2 1979598 forward nd [COMP-AINF] [1]
  promoter uspCp6 1979619 forward nd [COMP-AINF] [1]
  promoter uspCp5 1979658 forward nd [COMP-AINF] [1]
  promoter uspCp4 1979668 forward nd [COMP-AINF] [1]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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