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BirA Genetic Sensory Response Unit in Escherichia coli K-12 genome


Gensor Unit Map          
BioB BioD bioA BirA malonyl-[acp] methyl ester BioC alanine BioA oxidized [2Fe-2S] ferredoxin CO2 phosphate malonyl-S-ACP 9-mercaptodethiobiotin adenosylhomocysteine unsulfurated [sulfur donor] bioBFCD_mRNA BioF methionine biotin reduced [2Fe-2S] ferredoxin coenzyme A biotinyl-5'-AMP KAPA biotinyl-5'-AMP DAPA pimelyl-[acp] AcpP sulfurated sulfur donor dethiobiotin pimelyl-CoA bioA_mRNA 5'-deoxyadenosine bioBFCD SAM S-adenosyl-4-methylthio-2-oxobutanoate BirA alanine CO2 adenosylhomocysteine dethiobiotin SAM SAM 5'-deoxyadenosine


Gensor Unit       
Name: BirA
Description: Molecular biology level
Detailed: In the presence of biotin-5'-AMP, BirA negatively regulates the expression of genes required for biotin biosynthetic process. Under these conditions, BirA is modified to BirA-Biotynil-5-adenylate, binds its target sites preventing the expression of its regulated genes. In the absence of biotin-5'-AMP, BirA unbinds its target sites enabling the expression of its regulated genes.
Less detailed: In the presence of biotin-5'-AMP, BirA negatively regulates the expression of genes needed for in biotin biosynthetic process.

Physiology level
Detailed: The presence of biotin inhibits the expression of genes involved with biotin biosynthesis. Under these conditions the genes necessary for the conversion from malonyl-[ACP] through biotin are not expressed.
Less detailed: The presence of biotin-5'-AMP inhibits the expression of genes needful for the conversion from malonyl-ACP to biotin.

With enzymatic activity
Molecular biology level
Detailed: BirA is a dual-function protein, it is a transcriptional factor but also harbors enzymatic activity of biotin ligase. In the presence of biotin-5'-AMP BirA negatively regulates the expression of genes required for biotin biosynthetic process. Under these conditions, BirA is modified to BirA-Biotynil-5-adenylate, binds its target sites preventing (or avoiding or hindering) the expression of its regulated genes.
Less detailed: BirA dual-function protein is a transcriptional factor that also harbors enzymatic activity of biotin ligase.
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Classification:
Groups: Coenzyme transport and metabolism
Gene Ontology Terms (GO): biological_process GO:0009058 - biosynthetic process
GO:0009102 - biotin biosynthetic process
GO:0008152 - metabolic process
GO:0032259 - methylation
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0051537 - 2 iron, 2 sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0008710 - 8-amino-7-oxononanoate synthase activity
GO:0005524 - ATP binding
GO:0004015 - adenosylmethionine-8-amino-7-oxononanoate transaminase activity
GO:0004076 - biotin synthase activity
GO:0010340 - carboxyl-O-methyltransferase activity
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Components:
  Function Name Type
  simple_molecule 5'-deoxyadenosine simple_molecule
  simple_molecule 9-mercaptodethiobiotin simple_molecule
  simple_molecule AcpP simple_molecule
  protein BioA protein
  protein BioB protein
  protein BioC protein
  protein BioD protein
  protein BioF protein
  protein BirA protein
  complex BirA-biotinyl-5'-AMP complex
  simple_molecule CO2 simple_molecule
  simple_molecule DAPA simple_molecule
  simple_molecule KAPA simple_molecule
  simple_molecule S-adenosyl-4-methylthio-2-oxobutanoate simple_molecule
  simple_molecule SAM simple_molecule
  simple_molecule adenosylhomocysteine simple_molecule
  simple_molecule alanine simple_molecule
  gene bioA gene
  RNA bioA_mRNA RNA
  gene bioBFCD gene
  RNA bioBFCD_mRNA RNA
  simple_molecule biotin simple_molecule
  simple_molecule biotinyl-5'-AMP simple_molecule
  simple_molecule coenzyme A simple_molecule
  simple_molecule dethiobiotin simple_molecule
  simple_molecule malonyl-S-ACP simple_molecule
  simple_molecule malonyl-[acp] methyl ester simple_molecule
  simple_molecule methionine simple_molecule
  simple_molecule oxidized [2Fe-2S] ferredoxin simple_molecule
  simple_molecule phosphate simple_molecule
  simple_molecule pimelyl-CoA simple_molecule
  simple_molecule pimelyl-[acp] simple_molecule
  simple_molecule reduced [2Fe-2S] ferredoxin simple_molecule
  simple_molecule sulfurated sulfur donor simple_molecule
  simple_molecule unsulfurated [sulfur donor] simple_molecule


Reactions       
Showing 17 reactions
biotinyl-5'-AMP + BirA BirA-biotinyl-5'-AMP
Name: biotinyl-5'-AMP + BirA -> BirA-biotinyl-5'-AMP
Description: Reaction transforming biotinyl-5'-AMP, BirA into BirA-biotinyl-5'-AMP
Type: state transition
Pathway Components: biotinyl-5'-AMP + BirA -> BirA-biotinyl-5'-AMP
Components:
Function Name Type
reactant BirA protein
product BirA-biotinyl-5'-AMP complex
reactant biotinyl-5'-AMP simple_molecule
BirA-biotinyl-5'-AMP bioA bioA_mRNA
Name: bioA -> bioA_mRNA
Description: Transcription of transcription unit bioA. Reaction inhibition by BirA-biotinyl-5'-AMP.
Type: transcription
Pathway Components: bioA - BirA-biotinyl-5'-AMP -> bioA_mRNA
Components:
Function Name Type
inhibitor BirA-biotinyl-5'-AMP complex
reactant bioA gene
product bioA_mRNA RNA
BirA-biotinyl-5'-AMP bioBFCD bioBFCD_mRNA
Name: bioBFCD -> bioBFCD_mRNA
Description: Transcription of transcription unit bioBFCD. Reaction inhibition by BirA-biotinyl-5'-AMP.
Type: transcription
Pathway Components: bioBFCD - BirA-biotinyl-5'-AMP -> bioBFCD_mRNA
Components:
Function Name Type
inhibitor BirA-biotinyl-5'-AMP complex
reactant bioBFCD gene
product bioBFCD_mRNA RNA
bioBFCD_mRNA BioB
Name: bioBFCD_mRNA -> BioB
Description: Translation of BioB
Type: translation
Pathway Components: bioBFCD_mRNA -> BioB
Components:
Function Name Type
product BioB protein
reactant bioBFCD_mRNA RNA
5'-deoxyadenosine BioB adenosylhomocysteine methionine Reactants Products
Name: reduced [2Fe-2S] ferredoxin + sulfurated sulfur donor + dethiobiotin + SAM -> oxidized [2Fe-2S] ferredoxin + unsulfurated [sulfur donor] + 5'-deoxyadenosine + methionine + biotin
Description: Reaction transforming reduced [2Fe-2S] ferredoxin, sulfurated sulfur donor, dethiobiotin, SAM into oxidized [2Fe-2S] ferredoxin, unsulfurated [sulfur donor] , 5'-deoxyadenosine , methionine , biotin
Type: state transition
Pathway Components: reduced [2Fe-2S] ferredoxin + sulfurated sulfur donor + dethiobiotin + SAM - BioB - 5'-deoxyadenosine - adenosylhomocysteine - methionine -> oxidized [2Fe-2S] ferredoxin + unsulfurated [sulfur donor] + 5'-deoxyadenosine + methionine + biotin
Components: Show/Hide Components Table
BioB Reactants Products
Name: reduced [2Fe-2S] ferredoxin + sulfurated sulfur donor + dethiobiotin + SAM -> oxidized [2Fe-2S] ferredoxin + unsulfurated [sulfur donor] + 9-mercaptodethiobiotin + 5'-deoxyadenosine + methionine
Description: Reaction transforming reduced [2Fe-2S] ferredoxin, sulfurated sulfur donor, dethiobiotin, SAM into oxidized [2Fe-2S] ferredoxin, unsulfurated [sulfur donor] , 9-mercaptodethiobiotin , 5'-deoxyadenosine , methionine
Type: state transition
Pathway Components: reduced [2Fe-2S] ferredoxin + sulfurated sulfur donor + dethiobiotin + SAM - BioB -> oxidized [2Fe-2S] ferredoxin + unsulfurated [sulfur donor] + 9-mercaptodethiobiotin + 5'-deoxyadenosine + methionine
Components: Show/Hide Components Table
BioB 9-mercaptodethiobiotin + SAM 5'-deoxyadenosine + methionine + biotin
Name: 9-mercaptodethiobiotin + SAM -> 5'-deoxyadenosine + methionine + biotin
Description: Reaction transforming 9-mercaptodethiobiotin, SAM into 5'-deoxyadenosine, methionine , biotin
Type: state transition
Pathway Components: 9-mercaptodethiobiotin + SAM - BioB -> 5'-deoxyadenosine + methionine + biotin
Components:
Function Name Type
product 5'-deoxyadenosine simple_molecule
reactant 9-mercaptodethiobiotin simple_molecule
catalyzer BioB protein
reactant SAM simple_molecule
product biotin simple_molecule
product methionine simple_molecule
bioBFCD_mRNA BioF
Name: bioBFCD_mRNA -> BioF
Description: Translation of BioF
Type: translation
Pathway Components: bioBFCD_mRNA -> BioF
Components:
Function Name Type
product BioF protein
reactant bioBFCD_mRNA RNA
BioF pimelyl-[acp] + alanine KAPA + AcpP + CO2
Name: pimelyl-[acp] + alanine -> KAPA + AcpP + CO2
Description: Reaction transforming pimelyl-[acp], alanine into KAPA, AcpP , CO2
Type: state transition
Pathway Components: pimelyl-[acp] + alanine - BioF -> KAPA + AcpP + CO2
Components:
Function Name Type
product AcpP simple_molecule
catalyzer BioF protein
product CO2 simple_molecule
product KAPA simple_molecule
reactant alanine simple_molecule
reactant pimelyl-[acp] simple_molecule
BioF pimelyl-CoA + alanine coenzyme A + KAPA + CO2
Name: pimelyl-CoA + alanine -> coenzyme A + KAPA + CO2
Description: Reaction transforming pimelyl-CoA, alanine into coenzyme A, KAPA , CO2
Type: state transition
Pathway Components: pimelyl-CoA + alanine - BioF -> coenzyme A + KAPA + CO2
Components:
Function Name Type
catalyzer BioF protein
product CO2 simple_molecule
product KAPA simple_molecule
reactant alanine simple_molecule
product coenzyme A simple_molecule
reactant pimelyl-CoA simple_molecule
bioBFCD_mRNA BioC
Name: bioBFCD_mRNA -> BioC
Description: Translation of BioC
Type: translation
Pathway Components: bioBFCD_mRNA -> BioC
Components:
Function Name Type
product BioC protein
reactant bioBFCD_mRNA RNA
BioC malonyl-S-ACP + SAM malonyl-[acp] methyl ester + adenosylhomocysteine
Name: malonyl-S-ACP + SAM -> malonyl-[acp] methyl ester + adenosylhomocysteine
Description: Reaction transforming malonyl-S-ACP, SAM into malonyl-[acp] methyl ester, adenosylhomocysteine
Type: state transition
Pathway Components: malonyl-S-ACP + SAM - BioC -> malonyl-[acp] methyl ester + adenosylhomocysteine
Components:
Function Name Type
catalyzer BioC protein
reactant SAM simple_molecule
product adenosylhomocysteine simple_molecule
reactant malonyl-S-ACP simple_molecule
product malonyl-[acp] methyl ester simple_molecule
bioBFCD_mRNA BioD
Name: bioBFCD_mRNA -> BioD
Description: Translation of BioD
Type: translation
Pathway Components: bioBFCD_mRNA -> BioD
Components:
Function Name Type
product BioD protein
reactant bioBFCD_mRNA RNA
BioD DAPA + CO2 dethiobiotin + phosphate
Name: DAPA + CO2 -> dethiobiotin + phosphate
Description: Reaction transforming DAPA, CO2 into dethiobiotin, phosphate
Type: state transition
Pathway Components: DAPA + CO2 - BioD -> dethiobiotin + phosphate
Components:
Function Name Type
catalyzer BioD protein
reactant CO2 simple_molecule
reactant DAPA simple_molecule
product dethiobiotin simple_molecule
product phosphate simple_molecule
bioA_mRNA BioA
Name: bioA_mRNA -> BioA
Description: Translation of BioA
Type: translation
Pathway Components: bioA_mRNA -> BioA
Components:
Function Name Type
product BioA protein
reactant bioA_mRNA RNA
BioA KAPA KAPA + SAM S-adenosyl-4-methylthio-2-oxobutanoate + DAPA
Name: KAPA + SAM -> S-adenosyl-4-methylthio-2-oxobutanoate + DAPA
Description: Reaction transforming KAPA, SAM into S-adenosyl-4-methylthio-2-oxobutanoate, DAPA
Type: state transition
Pathway Components: KAPA + SAM - BioA - KAPA -> S-adenosyl-4-methylthio-2-oxobutanoate + DAPA
Components:
Function Name Type
catalyzer BioA protein
product DAPA simple_molecule
inhibitor KAPA simple_molecule
reactant KAPA simple_molecule
product S-adenosyl-4-methylthio-2-oxobutanoate simple_molecule
reactant SAM simple_molecule
malonyl-[acp] methyl ester pimelyl-[acp]
Name: malonyl-[acp] methyl ester -> pimelyl-[acp]
Description: Transformation of malonyl-[acp] methyl ester into pimelyl-[acp] by one or more reactions not shown.
Type: complementary pathway reaction
Pathway Components: malonyl-[acp] methyl ester / RXN-11474 / RXN-11476 / RXN-11477 / RXN-11478 / RXN-11479 / RXN-11480 / RXN-11481 / RXN-11482 / RXN-11483 / pimelyl-[acp]
Components:
Function Name Type
reactant malonyl-[acp] methyl ester simple_molecule
product pimelyl-[acp] simple_molecule
R1 R2 R3 R4 R5 R6 R7 R8 R9 R10 R11 R12 R13 R14 R15 R16 R17



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