RegulonDB RegulonDB 10.9: Operon Form
   

gcvA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: gcvA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: gcvA
Synonym(s): OP00233
Gene(s): gcvA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The protein H-NS positively regulates gcv operon expression, but this regulation seems to be indirect 11114904 Overproduction of BglG in stationary phase brings about downregulation of both gcvA and gcvB Shukla S,2019
The half-life of the gcvA mRNA is reduced by the RNA-binding protein BglG during stationary phase Harwani D,2012
Reference(s): [1] Jin T., et al., 1995
Promoter
Name: gcvAp
+1: 2942639
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 72
Sequence: aagtgccgccactataggtatttgctggtagaagctcaacggacaatttataatggctcaGattaaaaaaactaataggtt
                      -35                        -10        +1                   
Note(s): This promoter has another putative transcription start site. The two transcription start sites are spaced only 1 nt apart.
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[3] Wilson RL., et al., 1994
TF binding sites (TFBSs)
[GcvA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GcvA repressor gcvAp 2942624 2942638 9.0 taatggctcaGATTAAAAAAACTAATaggttacata nd [APIORCISFBSCS], [BCE], [GEA], [SM] [3], [4], [5], [6], [7]
proximal GcvA repressor gcvAp 2942646 2942660 -14.0 tagaagctcaACGGACAATTTATAATggctcagatt nd [AIBSCS], [APIORCISFBSCS], [BCE], [BPP], [GEA], [IC] [3], [4], [5], [6], [7]


Evidence    

 [HIPP] Human inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BCE] Binding of cellular extracts

 [GEA] Gene expression analysis

 [SM] Site mutation

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BPP] Binding of purified proteins

 [IC] Inferred by curator


Reference(s)    

 [1] Jin T., Inouye M., 1995, Identification of the genes in multicopy plasmids affecting ompC and ompF expression in Escherichia coli., FEMS Microbiol Lett 133(3):225-31

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Wilson RL., Stauffer GV., 1994, DNA sequence and characterization of GcvA, a LysR family regulatory protein for the Escherichia coli glycine cleavage enzyme system., J Bacteriol 176(10):2862-8

 [4] Jourdan AD., Stauffer GV., 1999, Genetic analysis of the GcvA binding site in the gcvA control region., Microbiology 145 ( Pt 8):2153-62

 [5] Stauffer LT., Stauffer GV., 2005, GcvA interacts with both the alpha and sigma subunits of RNA polymerase to activate the Escherichia coli gcvB gene and the gcvTHP operon., FEMS Microbiol Lett 242(2):333-8

 [6] Urbanowski ML., Stauffer LT., Stauffer GV., 2000, The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli., Mol Microbiol 37(4):856-68

 [7] Wilson RL., Urbanowski ML., Stauffer GV., 1995, DNA binding sites of the LysR-type regulator GcvA in the gcv and gcvA control regions of Escherichia coli., J Bacteriol 177(17):4940-6

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