RegulonDB RegulonDB 11.1: Operon Form
   

cynR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cynR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cynR
Synonym(s): OP00194
Gene(s): cynR   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Anderson PM., et al., 1990
Promoter
Name: cynRp
+1: 358709
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 19
Sequence: aggcagactactcgccgattgtcataaggtaaaagtctcatttatgatgagttccattggAtttacttataggttgcgaat
                                  -35                       +10                  
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [2] Huerta AM., et al., 2003
[3] Lamblin AF., et al., 1993
TF binding sites (TFBSs)
[CynR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CynR repressor cynRp 358700 358708 6.0 ttccattggaTTTACTTATaggttgcgaa nd [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] nd [4]
proximal CynR repressor cynRp 358738 358746 -33.0 gccgattgtcATAAGGTAAaagtctcatt nd [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] nd [4]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins


Reference(s)    

 [1] Anderson PM., Sung YC., Fuchs JA., 1990, The cyanase operon and cyanate metabolism., FEMS Microbiol Rev 7(3-4):247-52

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Lamblin AF., Fuchs JA., 1993, Expression and purification of the cynR regulatory gene product: CynR is a DNA-binding protein., J Bacteriol 175(24):7990-9

 [4] Lamblin AF., Fuchs JA., 1994, Functional analysis of the Escherichia coli K-12 cyn operon transcriptional regulation., J Bacteriol 176(21):6613-22

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