RegulonDB RegulonDB 10.9: Operon Form
   

flgAMN operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: flgAMN
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: flgMN
Gene(s): flgN, flgM   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: flgMp
+1: 1130165
Sigma Factor: Sigma28 Sigmulon
Distance from start of the gene: 37
Sequence: gaatattcttataaacctgtaagctgttaaagattacccgtcccttgccgataaataagcAacacatgataaaagcgccct
                            -35                -10          +1                   
Note(s): The sequence of this promoter has been defined, by computational analyses, from consensus sequence of the σ28F) promoters: TAAAGTTT in the -35 box and GCCGATAA in the -10 box Park K,2001. Ko M,2000. Ide N,1999. Therefore, we assigned a putative transcription start site for this promoter based on the observation that the majority of the σ28 promoters, determined experimentally, present an important -10 motif (5'-CGA-3') at the positions -12 to -10 and a distance of 6 nucleotides between the transcription start site and the -10 box 16855242. Yu HH,2006. Park K,2001.
Evidence: [HIPP]
[ICWHO]
Reference(s): [1] Chilcott GS., et al., 2000
[2] Huerta AM., et al., 2003
[3] Ide N., et al., 1999
[4] Ko M., et al., 2000
[5] Park K., et al., 2001
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CsgD repressor flgMp 1130142 1130156 17.5 caacacatgaTAAAAGCGCCCTCAAtgaggaataa nd [APIORCISFBSCS], [BPP] [6]
proximal CsgD repressor flgMp 1130158 1130172 1.5 cttgccgataAATAAGCAACACATGataaaagcgc nd [APIORCISFBSCS], [BPP] [6]


Transcription unit          
Name: flgAMN
Gene(s): flgN, flgM, flgA   Genome Browser M3D Gene expression COLOMBOS
Note(s): In a study where it was shown that the L-tryptophan eradicated persister cells, it was observed that the exogenous amino acid downregulated the expression of flgAMN as well as other flagellar genes 31271063.
Reference(s): [7] Macnab RM. 1992
Promoter
Name: flgAp
+1: 1130885
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 22
Sequence: cgcaaaatgggtcgctatttatgccgttgatggtcattgcggacaggtacaattcacgttGtagaaatggctgggggcgaa
                           -35                  -10         +1                   
Note(s): We assigned a putative transcriptional start site to this promoter based on the observation that the majority of the promoters, whose transcriptional start sites were determined experimentally, present a distance of 6 nucleotides between the transcriptional start site and the -10 box.
Evidence: [HIPP]
[ICWHO]
Reference(s): [2] Huerta AM., et al., 2003
[8] Stafford GP., et al., 2005
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FlhDC activator flgAp 1130917 1130933 -40.0 gcaaaatgggTCGCTATTTATGCCGTTGatggtcattg nd [APIORCISFBSCS], [GEA] [8]
proximal FlhDC activator flgAp 1130945 1130961 -68.0 ttacgcgcgcGTTATCGGCGGAATAAACgcaaaatggg nd [APIORCISFBSCS], [GEA] [8]




Reference(s)    

 [1] Chilcott GS., Hughes KT., 2000, Coupling of flagellar gene expression to flagellar assembly in Salmonella enterica serovar typhimurium and Escherichia coli., Microbiol Mol Biol Rev 64(4):694-708

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Ide N., Ikebe T., Kutsukake K., 1999, Reevaluation of the promoter structure of the class 3 flagellar operons of Escherichia coli and Salmonella., Genes Genet Syst 74(3):113-6

 [4] Ko M., Park C., 2000, Two novel flagellar components and H-NS are involved in the motor function of Escherichia coli., J Mol Biol 303(3):371-82

 [5] Park K., Choi S., Ko M., Park C., 2001, Novel sigmaF-dependent genes of Escherichia coli found using a specified promoter consensus., FEMS Microbiol Lett 202(2):243-50

 [6] Dudin O., Geiselmann J., Ogasawara H., Ishihama A., Lacour S., 2014, Repression of flagellar genes in exponential phase by CsgD and CpxR, two crucial modulators of Escherichia coli biofilm formation., J Bacteriol 196(3):707-15

 [7] Macnab RM., 1992, Genetics and biogenesis of bacterial flagella., Annu Rev Genet 26:131-58

 [8] Stafford GP., Ogi T., Hughes C., 2005, Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2., Microbiology 151(Pt 6):1779-88

 [9] Romilly C., Hoekzema M., Holmqvist E., Wagner EGH., 2020, Small RNAs OmrA and OmrB promote class III flagellar gene expression by inhibiting the synthesis of anti-Sigma factor FlgM., RNA Biol 17(6):872-880


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