RegulonDB RegulonDB 11.1: Operon Form
   

cusRS operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cusRS
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cusRS
Synonym(s): ylcA-ybcZ
Gene(s): cusS, cusR   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-TRANSCRIPT-LEN-DETERMINATION] Length of transcript experimentally determined
Reference(s): [1] Franke S., et al., 2001
Promoter
Name: cusRp
+1: 595462
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 19
Sequence: aaaattgtcattttgccaataagcgattgccatctgatcccgctactctagaattgcccgGgcaacatgcggaggaaatat
                                -35                   -10   +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [1] Franke S., et al., 2001
[2] Huerta AM., et al., 2003
[3] Yamamoto K., et al., 2005
TF binding sites (TFBSs)
[CusR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CusR-phosphorylated activator cusRp 595512 595528 -57.5 gtgacagaaaAATGACAAAATTGTCATtttgccaata nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [3], [4], [5]
[HprR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal HprR-phosphorylated activator cusRp 595511 595529 -57.5 ggtgacagaaAAATGACAAAATTGTCATTttgccaataa nd [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-GSELEX], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] nd [6]
[PhoB,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PhoB-phosphorylated activator cusRp 595510 595528 -57.0 gtgacagaaaAATGACAAAATTGTCATTTtgccaataag nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [7]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-GSELEX] high throughput Genomic SELEX-chip evidence


Reference(s)    

 [1] Franke S., Grass G., Nies DH., 2001, The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions., Microbiology 147(Pt 4):965-72

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Yamamoto K., Ishihama A., 2005, Transcriptional response of Escherichia coli to external copper., Mol Microbiol 56(1):215-27

 [4] Munson GP., Lam DL., Outten FW., O'Halloran TV., 2000, Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12., J Bacteriol 182(20):5864-71

 [5] Xu Z., Wang P., Wang H., Yu ZH., Au-Yeung HY., Hirayama T., Sun H., Yan A., 2019, Zinc excess increases cellular demand for iron and decreases tolerance to copper in Escherichia coli., J Biol Chem 294(45):16978-16991

 [6] Urano H., Umezawa Y., Yamamoto K., Ishihama A., Ogasawara H., 2015, Cooperative regulation of the common target genes between H2O2-sensing YedVW and Cu2+-sensing CusSR in Escherichia coli., Microbiology 161(Pt 4):729-38

 [7] Yang C., Huang TW., Wen SY., Chang CY., Tsai SF., Wu WF., Chang CH., 2012, Genome-wide PhoB binding and gene expression profiles reveal the hierarchical gene regulatory network of phosphate starvation in Escherichia coli., PLoS One 7(10):e47314

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