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| Operon |  |
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| Name: | dcuD |
| This page displays every known transcription unit of this operon and their known regulation. | |
| Name: | dcuD | |||||||||
| Gene(s): | dcuD Genome Browser M3D Gene expression COLOMBOS | |||||||||
| Note(s): | In spite of 0010525738 mentioning that the σ54 promoter consensus and the putative IHF site in the intergenic region were most likely part of the narR promoter, we believe that these correspond to the dcuC gene, based on two points: (1) the orientation of the putative σ54 sequence with the +1 site of the transcriptional start site in the dcuC direction and (2) the way that regulation (with the presence of the σ54 promoter type and IHF regulator at almost the same position and acting positively) is very similar to that of other promoters, such as glnHp2, pspAp, norVp, hycAp, hypAp, pspGp, and ibpBp, which are controlled by both factors. | |||||||||
| Evidence: | [AISGDTU] Automated inference that a single-gene directon is a transcription unit | |||||||||
| Promoter | ||||||||||
| Name: | dcuDp2 | |||||||||
| +1: | 3374583 | |||||||||
| Sigma Factor: | Sigma54 Sigmulon | |||||||||
| Distance from start of the gene: | 286 | |||||||||
| Sequence: |
acatcctatcacaggattgaaagtaggggaaaatggcagggttttctctttgtgcctcatCattaccataattaacggaat -24 -12 +1 |
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| Note(s): | We assigned a putative transcription start site to this promoter based on the observation that the majority of the σ54-dependent promoters, whose transcription start sites were determined experimentally, present a distance of 11 nucleotides between the transcription start site and the -12 box Barrios H,1999. | |||||||||
| Evidence: |
[HIPP] [ICWHO] |
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| Reference(s): |
[1] Huerta AM., et al., 2003 [2] Janausch IG., et al., 1999 |
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| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| proximal | IHF | activator | dcuDp2 | 3374533 | 3374545 | -44.0 | acatcctatcACAGGATTGAAAGTaggggaaaat | nd | [APIORCISFBSCS] | nd |
| Name: | dcuD | |||||||||
| Gene(s): | dcuD Genome Browser M3D Gene expression COLOMBOS | |||||||||
| Evidence: | [AISGDTU] Automated inference that a single-gene directon is a transcription unit | |||||||||
| Promoter | ||||||||||
| Name: | dcuDp | |||||||||
| +1: | 3374766 | |||||||||
| Sigma Factor: | Sigma70 Sigmulon | |||||||||
| Distance from start of the gene: | 103 | |||||||||
| Sequence: |
tacaaaataatcataagcgcatattttttaatgaaaaatcacctcacctacaattaaaaaCacgacatccgcaccataaat -35 -10 +1 |
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| Evidence: |
[HIPP] [ICWHO] |
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| Reference(s): | [1] Huerta AM., et al., 2003 | |||||||||
| Type | Transcription factor | Function | Promoter | Binding Sites | Growth Conditions | Evidence (Confirmed, Strong, Weak) | Reference(s) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| LeftPos | RightPos | Central Rel-Pos | Sequence | |||||||
| remote | PhoP-Phosphorylated1 | repressor | dcuDp | 3374812 | 3374832 | 56.5 | ataacatcgtTGTTTTCAATCTGCCGTTTATgggattgacc | nd | [AIBSCS], [GEA] | [3] |
| Note(s): |
1This regulatory interaction was identified by means of PhoPQ-related microarray experiments, and the analysis checked for the presence of the regulatory motif in the promoter region Monsieurs P,2005. A repressor function for dcuD gene transcription can be clearly anticipated from the localization of the PhoP-binding site, very close to the dcuDp promoter core region Monsieurs P,20051This regulatory interaction was identified by means of PhoPQ-related microarray experiments, and the analysis checked for the presence of the regulatory motif in the promoter region Monsieurs P,2005. A repressor function for dcuD gene transcription can be clearly anticipated from the localization of the PhoP-binding site, very close to the dcuDp promoter core region Monsieurs P,2005 |
| Reference(s) |
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|---|---|
[HIPP] Human inference of promoter position