RegulonDB RegulonDB 11.1: Operon Form
   

fiu operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: fiu
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: fiu
Gene(s): fiu   Genome Browser M3D Gene expression COLOMBOS
Note(s): Expression of fiu, along with that of other genes also regulated by Fur, is reduced under Zn excess Xu Z,2019.
Evidence: [COMP-AINF] Inferred computationally without human oversight
Promoter
Name: fiup
+1: 841648
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 117
Sequence: aaatgtatttctttttcgcgcaatgggtgatagaaaatcgctccaagtgataatgcttatCaaaattattatcactttcac
                                -35                     -10 +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
Reference(s): [1] Huerta AM., et al., 2003
[2] Newman DL., et al., 1999
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cyclic-AMP activator fiup 841796 841817 -158.5 agatgttcggTACTGCAACCATCTACAAATAAccccaccaac nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-SIMILAR-TO-CONSENSUS] W [4]
[Fur,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fur-Fe2+ repressor fiup 841628 841646 12.0 atgcttatcaAAATTATTATCACTTTCACgagcactatc nd [COMP-HINF-SIMILAR-TO-CONSENSUS] nd [2]
proximal Fur-Fe2+ repressor fiup 841637 841655 3.0 caagtgataaTGCTTATCAAAATTATTATcactttcacg nd [COMP-HINF-SIMILAR-TO-CONSENSUS] nd [2]
proximal Fur-Fe2+ repressor fiup 841638 841656 2.0 ccaagtgataATGCTTATCAAAATTATTAtcactttcac nd [COMP-HINF-SIMILAR-TO-CONSENSUS] nd [2]
proximal Fur-Fe2+ repressor fiup 841645 841663 -6.0 aatcgctccaAGTGATAATGCTTATCAAAattattatca nd [COMP-HINF-SIMILAR-TO-CONSENSUS] nd [2]
sRNA Interaction TU
sRNA TU Regulated Function Binding Sites Regulatory Mechanism Evidence (Confirmed, Strong, Weak) Reference(s)
PosLeft PosRight Target sequence (mRNA)
small regulatory RNA RybB fiu repressor 841601 841615 GCCACUGUUAAUCCC nd [EXP-IEP], [EXP-IMP-SITE-MUTATION] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-AINF-SIMILAR-TO-CONSENSUS] Automated inference based on similarity to consensus sequences

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IEP] Inferred from expression pattern

 [EXP-IMP-SITE-MUTATION] Site mutation


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Newman DL., Shapiro JA., 1999, Differential fiu-lacZ fusion regulation linked to Escherichia coli colony development., Mol Microbiol 33(1):18-32

 [3] Gogol EB., Rhodius VA., Papenfort K., Vogel J., Gross CA., 2011, Small RNAs endow a transcriptional activator with essential repressor functions for single-tier control of a global stress regulon., Proc Natl Acad Sci U S A 108(31):12875-80

 [4] Zhang Z., Gosset G., Barabote R., Gonzalez CS., Cuevas WA., Saier MH., 2005, Functional interactions between the carbon and iron utilization regulators, Crp and Fur, in Escherichia coli., J Bacteriol 187(3):980-90

RegulonDB