RegulonDB RegulonDB 10.9: Operon Form
   

metJ operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: metJ
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: metJ
Gene(s): metJ   Genome Browser M3D Gene expression COLOMBOS
Note(s): There is a site for a MetJ regulator at -164.5 upstream from the start site of translation of the metJ gene. This gene has three promoters, and Saint-Girons I,1984did not mention which of them is regulated by MetJ, a protein that autoregulates by repression its own regulation.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Saint-Girons I., et al., 1984
[2] Smith AA., et al., 1984
Promoter
Name: metJp3
+1: 4128466
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 71
Sequence: atcacgccgacaggtgcattacacgatgtcacggtaacgcctgtacggtaaactatgcggGtttacggtcagtacccacat
                          -35                    -10        +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [3] Huerta AM., et al., 2003
[4] Kirby TW., et al., 1986


Transcription unit          
Name: metJ
Gene(s): metJ   Genome Browser M3D Gene expression COLOMBOS
Note(s): There is a site for a MetJ regulator at -164.5 upstream from the start site of translation of the metJ gene. This gene has three promoters, and Saint-Girons I,1984did not mention which of them is regulated by MetJ, a protein that autoregulates by repression its own regulation.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Saint-Girons I., et al., 1984
[2] Smith AA., et al., 1984
Promoter
Name: metJp2
+1: 4128529
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 134
Sequence: catctaaacttctttgcgtatagattgagcaaatcccaaatagccgttaaaattatatgcAttatcacgccgacaggtgca
                         -35                    -10         +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [3] Huerta AM., et al., 2003
[4] Kirby TW., et al., 1986
TF binding sites (TFBSs)
[Fur,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fur-Fe2+ repressor metJp2 4128521 4128539 -1.0 atagccgttaAAATTATATGCATTATCACGccgacaggtg nd [AIBSCS] [5]


Transcription unit       
Name: metJ
Gene(s): metJ   Genome Browser M3D Gene expression COLOMBOS
Note(s): There is a site for a MetJ regulator at -164.5 upstream from the start site of translation of the metJ gene. This gene has three promoters, and Saint-Girons I,1984did not mention which of them is regulated by MetJ, a protein that autoregulates by repression its own regulation.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Saint-Girons I., et al., 1984
[2] Smith AA., et al., 1984
Promoter
Name: metJp1
+1: 4128558
Distance from start of the gene: 163
Sequence: ttgtgttaatggacgtcaatacatctggacatctaaacttctttgcgtatagattgagcaAatcccaaatagccgttaaaa
Evidence: [TIM]
Reference(s): [4] Kirby TW., et al., 1986


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences


Reference(s)    

 [1] Saint-Girons I., Duchange N., Cohen GN., Zakin MM., 1984, Structure and autoregulation of the metJ regulatory gene in Escherichia coli., J Biol Chem 259(22):14282-5

 [2] Smith AA., Greene RC., 1984, Cloning of the methionine regulatory gene, metJ, of Escherichia coli K12 and identification of its product., J Biol Chem 259(22):14279-81

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Kirby TW., Hindenach BR., Greene RC., 1986, Regulation of in vivo transcription of the Escherichia coli K-12 metJBLF gene cluster., J Bacteriol 165(3):671-7

 [5] Stojiljkovic I., Baumler AJ., Hantke K., 1994, Fur regulon in gram-negative bacteria. Identification and characterization of new iron-regulated Escherichia coli genes by a fur titration assay., J Mol Biol 236(2):531-45

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