RegulonDB RegulonDB 10.9: Operon Form
   

cueO operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cueO
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: cueO
Gene(s): cueO   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: cueOp
+1: 137045
Distance from start of the gene: 38
Sequence: tcgattaaattgtgtctgcggcttgaccttcccgtaaggggaaggactatgctcaacgttTgattttgtttcgcctgctta
Note(s): This promoter has a putative transcription start site.
Evidence: [HIPP]
[RS-EPT-CBR]
Reference(s): [1] Outten FW., et al., 2000
[2] Salgado H, et al., 2012
TF binding sites (TFBSs)
[CueR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CueR-Cu+1 activator cueOp 137011 137029 -25.0 tgcggcttgaCCTTCCCGTAAGGGGAAGGActatgctcaa nd [APIORCISFBSCS], [BPP], [GEA] [1], [3], [4]
Note(s): 1The presence of Fe(III) provokes the dissociation of CueR from the DNA binding site upstream of cueO Xu Z,2019.1The presence of Fe(III) provokes the dissociation of CueR from the DNA binding site upstream of cueO Xu Z,2019.


Evidence    

 [HIPP] Human inference of promoter position

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis


Reference(s)    

 [1] Outten FW., Outten CE., Hale J., O'Halloran TV., 2000, Transcriptional activation of an Escherichia coli copper efflux regulon by the chromosomal MerR homologue, cueR., J Biol Chem 275(40):31024-9

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Xu Z., Wang P., Wang H., Yu ZH., Au-Yeung HY., Hirayama T., Sun H., Yan A., 2019, Zinc excess increases cellular demand for iron and decreases tolerance to copper in Escherichia coli., J Biol Chem 294(45):16978-16991

 [4] Yamamoto K., Ishihama A., 2005, Transcriptional response of Escherichia coli to external copper., Mol Microbiol 56(1):215-27

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