RegulonDB RegulonDB 10.8: Operon Form
   

grxA operon and associated TUs in Escherichia coli K-12 genome


Transcription unit          
Name: grxA
Gene(s): grxA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The transcription of the gene grxA is enhanced under high oxygen saturation (300%) in the absence of the superoxide dismutase proteins SodA and SodB Baez A,2013
Sulfane sulfur modifies OxyR at Cys199 to form a protein persulfide that in turn activates the expression of grxA, trxC, and katG promoters under both aerobic and anaerobic conditions 31421411
The transcript of grxA is increased after σE induction, as observed in high-throughput analysis of gene expression Lacoux C,2020.
Reference(s): [1] Tao K. 1997
Promoter
Name: grxAp
+1: 890776
Distance from start of the gene: 23
Sequence: attaatgcaacaggttaaacctactttcagcgaatacattttagcgtgatcattacaggcAtaaatctatgaggagagaaa
Note(s): This promoter appears to be induced in two phases after σE induction. It is induced during the first 5 minutes following σE induction, decreased from 5 to 10 minutes, induced from 10 to 15 minutes, and decreased from 15 to 20 minutes Lacoux C,2020.
Evidence: [TIM]
Reference(s): [1] Tao K. 1997
TF binding sites (TFBSs)
[OxyR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal OxyR activator grxAp 890761 890777 8.0 tcattacaggCATAAATCTATGAGGAGagaaataatg nd , [IHBCE], [2]
proximal OxyR activator grxAp 890812 890828 -44.0 caattaatgcAACAGGTTAAACCTACTttcagcgaat [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [1], [3]
proximal OxyR activator grxAp 890834 890850 -66.0 gtttaacagtTATAGCTTTTAGCAATTaatgcaacag [APIORCISFBSCS], [BPP], , [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA], [IHBCE], [1], [2], [3]


Evidence    

 [TIM] Transcription initiation mapping

 [CEUMA] ChIP-chip evidence used in manual assertion

 [IHBCE] Inferred by a human based on computational evidence

 [RE] RNA-seq evidence

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins

 [CV(CHIP-SV/GEA/ROMA)] cross validation(CHIP-SV/GEA/ROMA)

 [CV(GEA/ROMA)] cross validation(GEA/ROMA)

 [GEA] Gene expression analysis


Reference(s)    

 [1] Tao K., 1997, oxyR-dependent induction of Escherichia coli grx gene expression by peroxide stress., J Bacteriol 179(18):5967-70

 [2] Seo SW., Kim D., Szubin R., Palsson BO., 2015, Genome-wide Reconstruction of OxyR and SoxRS Transcriptional Regulatory Networks under Oxidative Stress in Escherichia coli K-12 MG1655., Cell Rep 12(8):1289-99

 [3] Zheng M., Wang X., Templeton LJ., Smulski DR., LaRossa RA., Storz G., 2001, DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide., J Bacteriol 183(15):4562-70

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