RegulonDB RegulonDB 11.1: Operon Form
   

grxA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: grxA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: grxA
Gene(s): grxA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The transcription of the gene grxA is enhanced under high oxygen saturation (300%) in the absence of the superoxide dismutase proteins SodA and SodB Baez A,2013
Sulfane sulfur modifies OxyR at Cys199 to form a protein persulfide that in turn activates the expression of grxA, trxC, and katG promoters under both aerobic and anaerobic conditions Hou N, Yan Z, Fan K, Li H, Zhao R, Xia Y, Xun L, Liu H,2019
The transcript of grxA is increased after σE induction, as observed in high-throughput analysis of gene expression Lacoux C,2020.
Reference(s): [1] Tao K. 1997
Promoter
Name: grxAp
+1: 890776
Distance from start of the gene: 23
Sequence: attaatgcaacaggttaaacctactttcagcgaatacattttagcgtgatcattacaggcAtaaatctatgaggagagaaa
Note(s): This promoter appears to be induced in two phases after σE induction. It is induced during the first 5 minutes following σE induction, decreased from 5 to 10 minutes, induced from 10 to 15 minutes, and decreased from 15 to 20 minutes Lacoux C,2020.
Evidence: [EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [1] Tao K. 1997
TF binding sites (TFBSs)
[OxyR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal OxyR activator grxAp 890761 890777 8.0 tcattacaggCATAAATCTATGAGGAGagaaataatg nd [EXP-IEP-RNA-SEQ], [COMP-HINF], [EXP-CHIP-CHIP-MANUAL] S [2]
proximal OxyR activator grxAp 890812 890828 -44.0 caattaatgcAACAGGTTAAACCTACTttcagcgaat nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [1], [3]
proximal OxyR activator grxAp 890834 890850 -66.0 gtttaacagtTATAGCTTTTAGCAATTaatgcaacag nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IEP-RNA-SEQ], [COMP-HINF], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-CHIP-EXO-MANUAL], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] C [1], [2], [3]


Evidence    

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [EXP-IEP-RNA-SEQ] RNA-seq evidence

 [COMP-HINF] Inferred by a human based on computational evidence

 [EXP-CHIP-CHIP-MANUAL] ChIP-chip evidence used in manual assertion

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-CHIP-EXO-MANUAL] ChIP-exo evidence used in manual assertion


Reference(s)    

 [1] Tao K., 1997, oxyR-dependent induction of Escherichia coli grx gene expression by peroxide stress., J Bacteriol 179(18):5967-70

 [2] Seo SW., Kim D., Szubin R., Palsson BO., 2015, Genome-wide Reconstruction of OxyR and SoxRS Transcriptional Regulatory Networks under Oxidative Stress in Escherichia coli K-12 MG1655., Cell Rep 12(8):1289-99

 [3] Zheng M., Wang X., Templeton LJ., Smulski DR., LaRossa RA., Storz G., 2001, DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide., J Bacteriol 183(15):4562-70

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