RegulonDB RegulonDB 11.1: Operon Form

argX-hisR-leuT-proM operon and associated TUs in Escherichia coli K-12 genome

Name: argX-hisR-leuT-proM
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: argX-hisR-leuT-proM
Synonym(s): argX
Gene(s): argX, hisR, leuT, proM   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
[EXP-IDA-TRANSCRIPT-LEN-DETERMINATION] Length of transcript experimentally determined
Reference(s): [1] Ow MC., et al., 2002
Name: argXp
+1: 3982364
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 11
Sequence: gcaatttatttaaaaagggactagacagaggggtgggaagtccgtattatccacccccgcAacggcgctaagcgcccgtag
                      -35                    -10            +1                   
Note(s): argXp promoter activity is inhibited by the stringent response in vivo Rowley KB,1993.
The transcription of the argXp promoter, also called the argTp promoter, is reduced by guanosine tetraphosphate (ppGpp), which modifies the RNA polymerase that forms an inactive promoter complex Maitra A,2005. Toulokhonov II,2001.
Individually, DksA and ppGpp activate and inhibit, respectively, the transcription started from the argX promoter (argXp). On the other hand, argXp also is regulated negatively by DksA, and this response occurs in the absence of ppGpp Lyzen R,2016
Reference(s): [2] Maitra A., et al., 2005
Type: rho-independent
Reference(s): [3] Hsu LM., et al., 1984
[4] Peters JM., et al., 2009
[5] Zuker M. 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis activator argXp 3982282 3982296 -75.0 agcagcaaaaCGCACAAACCGTAACcaaacgcgca nd [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] nd nd
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  DksA activation argXp nd   [EXP-IDA] [6]
  DksA-ppGpp inhibition argXp nd   [EXP-IDA] [6]
  ppGpp inhibition argXp nd   [EXP-IDA] [6]
Evidence: [EXP-IDA] Inferred from direct assay
Reference(s): [6] Lyzen R., et al., 2016