RegulonDB RegulonDB 10.9: Operon Form

metT-leuW-glnUW-metU-glnVX operon and associated TUs in Escherichia coli K-12 genome

Name: metT-leuW-glnUW-metU-glnVX
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: metT-leuW-glnUW-metU-glnVX
Synonym(s): metT
Gene(s): glnX, glnV, metU, glnW, glnU, leuW, metT   Genome Browser M3D Gene expression COLOMBOS
Note(s): This operon overlaps with three ORFs in the opposite direction.
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Mohanty BK., et al., 2008
[2] Nakajima N., et al., 1981
[3] Nakajima N., et al., 1982
Name: metTp
+1: 697163
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 30
Sequence: caattttccttgaaaaagaggttgacgctgcaaggctctatacgcataatgcgccccgcaAcgccgataaggtatcgcgaa
                      -35                    -10            +1                   
Evidence: [HIPP]
Reference(s): [4] Huerta AM., et al., 2003
Type: rho-independent
Reference(s): [5] Fournier MJ., et al., 1985
[6] Peters JM., et al., 2009
[7] Zuker M. 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis activator metTp 697223 697237 -67.0 acctaaccaaACAGTCACTTTCGAGCaattttcctt nd [IHBCE] nd
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp inhibition metTp nd Based on results of nuclease protection and primer extension assays, leuV, argT, metT, and leuX were determined to be involved in the stringent response induced by serine hydroxamate Rowley KB,1993. [IEP] [8]
Evidence: [IEP] Inferred from expression pattern
Reference(s): [8] Rowley KB., et al., 1993

Transcription unit       
Name: glnW-metU-glnVX
Gene(s): glnX, glnV, metU, glnW   Genome Browser M3D Gene expression COLOMBOS
Name: glnWp
+1: Unknown
Note(s): Zaslaver et al. demonstrated in 2006, by means of a library of fluorescent transcription fusions, that this promoter can be transcribed in vitro Zaslaver A,2006. Based on this, a putative promoter was suggested, but the +1 site of the transcription initiation has not been determined, although there exists promoter activity.
Evidence: [IEP]
Reference(s): [9] Zaslaver A., et al., 2006
Type: rho-independent
Reference(s): [5] Fournier MJ., et al., 1985
[6] Peters JM., et al., 2009
[7] Zuker M. 2003


 [1] Mohanty BK., Kushner SR., 2008, Rho-independent transcription terminators inhibit RNase P processing of the secG leuU and metT tRNA polycistronic transcripts in Escherichia coli., Nucleic Acids Res 36(2):364-75

 [2] Nakajima N., Ozeki H., Shimura Y., 1981, Organization and structure of an E. coli tRNA operon containing seven tRNA genes., Cell 23(1):239-49

 [3] Nakajima N., Ozeki H., Shimura Y., 1982, In vitro transcription of the supB-E tRNA operon of Escherichia coli. Characterization of transcription products., J Biol Chem 257(18):11113-20

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [5] Fournier MJ., Ozeki H., 1985, Structure and organization of the transfer ribonucleic acid genes of Escherichia coli K-12., Microbiol Rev 49(4):379-97

 [6] Peters JM., Mooney RA., Kuan PF., Rowland JL., Keles S., Landick R., 2009, Rho directs widespread termination of intragenic and stable RNA transcription., Proc Natl Acad Sci U S A 106(36):15406-11

 [7] Zuker M., 2003, Mfold web server for nucleic acid folding and hybridization prediction., Nucleic Acids Res 31(13):3406-15

 [8] Rowley KB., Elford RM., Roberts I., Holmes WM., 1993, In vivo regulatory responses of four Escherichia coli operons which encode leucyl-tRNAs., J Bacteriol 175(5):1309-15

 [9] Zaslaver A., Bren A., Ronen M., Itzkovitz S., Kikoin I., Shavit S., Liebermeister W., Surette MG., Alon U., 2006, A comprehensive library of fluorescent transcriptional reporters for Escherichia coli., Nat Methods 3(8):623-8