RegulonDB RegulonDB 10.9: Operon Form
   

uxuAB operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: uxuAB
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: uxuAB
Gene(s): uxuA, uxuB   Genome Browser M3D Gene expression COLOMBOS
Note(s): uxuAB were found to be downregulated in a MG1655 lysogen carrying the Stx2a phage PA8 1208335|.
Evidence: [IEP] Inferred from expression pattern
[ITCR] Inferred through co-regulation
Reference(s): [1] Blanco C., et al., 1986
Promoter
Name: uxuAp
+1: 4551518
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 118
Sequence: tgtggttaaccaatttcagaattcgggttgacatgtcttaccaaaaggtagaacttatacGccatctcatccgatgcaacg
                            -35                  -10        +1                   
Note(s): The Pribnow box of the uxuA promoter shows poor homology with the consensus sequence but contains the two most conserved nucleotides: the A in the second position and the T in the last position. The spacer length between this sequence and the -35 region is 17 nucleotides, the optimal distance for promoter efficiency.
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[3] Lynch AS., et al., 1996
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP activator uxuAp 4551365 4551386 -142.5 ggtcaaccatTGTTGCGATGAATGTCACATCCTctgatcaata nd [AIBSCS], [GEA] nd
proximal CRP-cAMP activator uxuAp 4551450 4551471 -57.5 agagtgaaatTCTTGTGATGTGGTTAACCAATTtcagaattcg nd [APIORCISFBSCS], [BPP] [1]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote ExuR repressor uxuAp 4551350 4551367 -159.5 tatgttatgtAAATTGGTCAACCATTGTTgcgatgaatg nd [APIORCISFBSCS], [BPP], [GEA], [SM] [4]
proximal ExuR repressor uxuAp 4551456 4551473 -53.5 aaattcttgtGATGTGGTTAACCAATTTCagaattcggg nd [APIORCISFBSCS], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal OxyR repressor uxuAp 4551456 4551472 -54.0 aaattcttgtGATGTGGTTAACCAATTTcagaattcgg nd [APIORCISFBSCS], [GEA] [5]
proximal OxyR repressor uxuAp 4551478 4551494 -32.0 caatttcagaATTCGGGTTGACATGTCTtaccaaaagg nd [APIORCISFBSCS], [GEA] [5]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote UxuR repressor uxuAp 4551350 4551367 -159.5 tatgttatgtAAATTGGTCAACCATTGTTgcgatgaatg nd [APIORCISFBSCS], [BPP], [GEA], [SM] nd
proximal UxuR repressor uxuAp 4551456 4551473 -53.5 aaattcttgtGATGTGGTTAACCAATTTCagaattcggg nd [APIORCISFBSCS], [GEA] [4], [6], [7]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -9.7 4551595 4551628 ggttccgcgtCTCTTTGCTGTGGAACCCACTATGTGAAAGAGGaaaaatcatg
  terminator -9.6 4552859 4552894 ggttccttctTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGtttacgatga
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





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