RegulonDB RegulonDB 10.9: Operon Form
   

ampC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: ampC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: ampC
Gene(s): ampC   Genome Browser M3D Gene expression COLOMBOS
Note(s): A transcriptional attenuator sequence that regulates the ampC gene expression was identified downstream of the ampCp promoter Jaurin B,1981. 17326753
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Bishop RE., et al., 1995
[2] Jaurin B., et al., 1981
[3] Jaurin B., et al., 1982
Promoter
Name: ampCp
+1: 4379003
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 59
Sequence: tacggtctggctgctatcctgacagttgtcacgctgattggtgtcgttacaatctaacgcAtcgccaatgtaaatccggcc
                          -35                   -10         +1                   
Evidence: [ICWHO]
[IMP]
[RS-EPT-CBR]
Reference(s): [4] Huerta AM., et al., 2003
[2] Jaurin B., et al., 1981
[3] Jaurin B., et al., 1982
[5] Salgado H, et al., 2012
Terminator(s)
Type: rho-independent
Sequence: gcggagaaaaGGTCCGAAAATTCGGACCcgatggaatt
Reference(s): [1] Bishop RE., et al., 1995
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd BolA-phosphorylated1 activator ampCp nd nd nd nd nd [BPP], [GEA], [IGI] [6]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd BolA-phosphorylated1 repressor ampCp nd nd nd nd nd [BPP], [GEA], [IGI] [6]
Note(s): 1Galego et al. demonstrated that the phosphorylation of BolA is essential for its activity as a transcriptional regulator; they demonstrated this using a promoter of the mreB gene, which is regulated by this protein Galego L,2020.1Galego et al. demonstrated that the phosphorylation of BolA is essential for its activity as a transcriptional regulator; they demonstrated this using a promoter of the mreB gene, which is regulated by this protein Galego L,2020.1Galego et al. demonstrated that the phosphorylation of BolA is essential for its activity as a transcriptional regulator; they demonstrated this using a promoter of the mreB gene, which is regulated by this protein Galego L,2020.
2Galego et al. demonstrated that the phosphorylation of BolA is essential for its activity as a transcriptional regulator; they demonstrated this using a promoter of the mreB gene, which is regulated by this protein Galego L,2020.


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -19.1 4378961 4378987 aatgtaaatcCGGCCCGCCTATGGCGGGCCGTTTTGtatggaaacc
  anti-terminator -12.51 4378984 4379031 cagttgtcacGCTGATTGGTGTCGTTACAATCTAACGCATCGCCAATGTAAATCCGGcccgcctatg
  anti-anti-terminator -8.6 4379028 4379061 gggttttctaCGGTCTGGCTGCTATCCTGACAGTTGTCACGCTgattggtgtc
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"




Reference(s)    

 [1] Bishop RE., Penfold SS., Frost LS., Holtje JV., Weiner JH., 1995, Stationary phase expression of a novel Escherichia coli outer membrane lipoprotein and its relationship with mammalian apolipoprotein D. Implications for the origin of lipocalins., J Biol Chem 270(39):23097-103

 [2] Jaurin B., Grundstrom T., Edlund T., Normark S., 1981, The E. coli beta-lactamase attenuator mediates growth rate-dependent regulation., Nature 290(5803):221-5

 [3] Jaurin B., Grundstrom T., Normark S., 1982, Sequence elements determining ampC promoter strength in E. coli., EMBO J 1(7):875-81

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Santos JM., Lobo M., Matos AP., De Pedro MA., Arraiano CM., 2002, The gene bolA regulates dacA (PBP5), dacC (PBP6) and ampC (AmpC), promoting normal morphology in Escherichia coli., Mol Microbiol 45(6):1729-40


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