RegulonDB RegulonDB 10.9: Operon Form
   

hupA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: hupA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: hupA
Gene(s): hupA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[LTED] Length of transcript experimentally determined
Reference(s): [1] Kohno K., et al., 1990
Promoter
Name: hupAp
+1: 4200176
Distance from start of the gene: 105
Sequence:
Note(s): The activity of this promoter appears to be decreased in the presence of σE. It is repressed during the first 5 minutes following σE induction and decreased from 5 to 10 minutes and from 10 to 20 minutes after induction of the σ factor Lacoux C,2020.
Single-cell assays have shown that the hupAp promoter is heterogeneously activated in cells within the same culture. In some cells the promoter is activated to a greater extent than in others; in approximately 20% of the cells in a given culture the promoter is not activated 20964794
Evidence: [HIPP]
[RS-EPT-CBR]
[TIM]
Reference(s): [2] Claret L., et al., 1996
[1] Kohno K., et al., 1990
[3] Salgado H, et al., 2012
Terminator(s)
Type: rho-independent
Sequence: agttttaacgAAGGGGTGGTTTCACCCCTTttgtctttct
Reference(s): [1] Kohno K., et al., 1990
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator hupAp 4200102 4200123 -63.5 taagaccagaAAACGTGATTTAACGCCTGATTTgtcgtacctg nd [BPP], [GEA] [2]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Fis activator hupAp 4199914 4199928 -255.0 aggctggtcgCGAAATGAGCGATGAAgagcttaaag nd [BPP], [GEA] [2]
remote Fis activator hupAp 4199949 4199963 -219.5 gaaaacccagCTGTAGAGCAAGAATGggacattcag nd [BPP], [GEA] [2]
remote Fis activator hupAp 4200001 4200015 -167.5 ttttagctgaGTGCGAAGAACGCGATatcgagctga nd [BPP], [GEA] [2]
remote Fis activator hupAp 4200075 4200089 -93.5 agcaatattgGTATAATTTTTCAGCAataagaccag nd [BPP], [GEA] [2]





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