RegulonDB RegulonDB 10.8: Operon Form
   

cfa operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: cfa
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: cfa
Synonym(s): cdfA
Gene(s): cfa   Genome Browser M3D Gene expression COLOMBOS
Note(s): Both promoters cfap2 (σ38) and cfap1 (σ38 and σ70) are induced in response to neutral acetate Rosenthal AZ,2006. 18331469 and they do not need accessory elements (activators or repressors), which suggests that acetate works directly with RNA polymerase in the transcription complex. The general responses to acetate of the two RNA polymerase forms were clearly different; σ70 promoters responded with wide differences, but σ38 promoters were generally activated 18331469
cfa transcription is also induced by acidic and osmotic stress 11244055. Weber H,2005 cfap1 contains a perfect 14-bp inverted repeat with two half-site elements that read 5'-TTTTGTT-3', centered at position -40. No information concerning this inverted repeat is available, since it does not align with any known regulator-binding sites 18331469
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Wang AY., et al., 1994
Promoter
Name: cfap1
+1: 1741201
Sigma Factor: Sigma38, Sigma70
Distance from start of the gene: 212
Sequence: tacttaggctgctaacaaaattttgttgtatgattgaaattagcggcctatactaatttcGagttgttaaagctacgataa
                          -35                    -10        +1                   
Evidence: [CV(RS-EPT-CBR/TA)]
[CV(TA/TIM)]
[HIPP]
[RS-EPT-CBR]
[TIM]
Reference(s): [2] Kvint K., et al., 2000
[3] Salgado H, et al., 2012
[1] Wang AY., et al., 1994
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  ppGpp activation cfap1   [GEA] [2]
Evidence: [GEA] Gene expression analysis
Reference(s): [2] Kvint K., et al., 2000


Transcription unit       
Name: cfa
Synonym(s): cdfA
Gene(s): cfa   Genome Browser M3D Gene expression COLOMBOS
Note(s): Both promoters cfap2 (σ38) and cfap1 (σ38 and σ70) are induced in response to neutral acetate Rosenthal AZ,2006. 18331469 and they do not need accessory elements (activators or repressors), which suggests that acetate works directly with RNA polymerase in the transcription complex. The general responses to acetate of the two RNA polymerase forms were clearly different; σ70 promoters responded with wide differences, but σ38 promoters were generally activated 18331469
cfa transcription is also induced by acidic and osmotic stress 11244055. Weber H,2005 cfap1 contains a perfect 14-bp inverted repeat with two half-site elements that read 5'-TTTTGTT-3', centered at position -40. No information concerning this inverted repeat is available, since it does not align with any known regulator-binding sites 18331469
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Wang AY., et al., 1994
Promoter
Name: cfap2
+1: 1741379
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 34
Sequence: agactcgcggttttttctgcgagatttctcacaaagcccaaaaagcgtctacgctgttttAaggttctgatcaccgaccag
                            -35              -10            +1                   
Note(s): Region 4 of the σ38 protein and the elements near the -35 DNA region are involved in the response to high acetate concentrations, which ensures the transcriptional activation of the cfap2 promoter to counteract these physiological changes Rosenthal AZ,2006.
Evidence: [HIPP]
[TIM]
Reference(s): [1] Wang AY., et al., 1994





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