RegulonDB RegulonDB 10.10: Operon Form
   

pheLA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: pheLA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: pheLA
Gene(s): pheL, pheA   Genome Browser M3D Gene expression COLOMBOS
Note(s): The expression of the gene pheA is diminished under oxygen limitation Kawaguchi H, Miyagawa H, Nakamura-Tsuruta S, Takaya N, Ogino C, Kondo A,2019.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Hudson GS., et al., 1984
Promoter
Name: pheLp
+1: 2737579
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 20
Sequence: aacgcgccttcgggcgcgttttttgttgacagcgtgaaaacagtacgggtactgtactaaAgtcacttaaggaaacaaaca
                          -35                     -10       +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [1] Hudson GS., et al., 1984
[2] Huerta AM., et al., 2003
Terminator(s)
Type: rho-independent
Sequence: ctgatgaaaaGGTGCCGGATGATGTGAATCATCCGGCACtggattatta
Reference(s): [1] Hudson GS., et al., 1984


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -16.7 2737519 2737545 tgtatcgccaACGCGCCTTCGGGCGCGTTTTTTGTTgacagcgtga
  terminator -18.6 2737693 2737724 acgaacaataAGGCCTCCCAAATCGGGGGGCCTTTTTTATTgataacaaaa
  anti-terminator -21.9 2737648 2737703 tcccctgaatGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAaatcgggggg
  anti-anti-terminator -14.44 2737620 2737670 ccgtttttctTCGCATTCTTTTTTACCTTCCCCTGAATGGGAGGCGTTTCGTCGTGTGAAacagaatgcg
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"
REGULATION, RNA cis-regulatory element:  
Rfam type: Cis-reg; leader
Strand: forward
Evidence: [ICA] Inferred by computational analysis
Reference(s): [4] null null
  Description Rfam score Left Pos Right Pos Sequence (RNA-strand)
  Phenylalanine leader peptide 130.8 2737598 2737723 aggaaacaaaCAUGAAACACAUACCGUUUUUCUUCGCAUUCUUUUUUACCUUCCCCUGAAUGGGAGGCGUUUCGUCGUGUGAAACAGAAUGCGAAGACGAACAAUAAGGCCUCCCAAAUCGGGGGGCCUUUUUUAUugauaacaaa
Notes: "The provied "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appears as the reverse complement of the sequence delimited by LeftPos-RightPos"


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping


Reference(s)    

 [1] Hudson GS., Davidson BE., 1984, Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12., J Mol Biol 180(4):1023-51

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] King AM., Vanderpool CK., Degnan PH., 2019, sRNA Target Prediction Organizing Tool (SPOT) Integrates Computational and Experimental Data To Facilitate Functional Characterization of Bacterial Small RNAs., mSphere 4(1)

 [4] null, null, null, null


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