RegulonDB RegulonDB 10.8: Operon Form
   

rplM-rpsI operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: rplM-rpsI
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: rplM-rpsI
Gene(s): rpsI, rplM   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Huerta AM., et al., 2003
[2] Isono S., et al., 1985
[3] Kaczanowska M., et al., 2004
Promoter
Name: rplMp
+1: 3378672
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 21
Sequence: cggactttgtcgtgtgaacctcaacaattgaagacgtttgggtgttcaccaacgtgtaacTatttattgggtaagctttta
                            -35                   -10       +1                   
Evidence: [AIPP]
Reference(s): [1] Huerta AM., et al., 2003
Terminator(s)
Type: rho-independent
Sequence: ttttagctatAAAAAAACCCGCCGAAGCGGGTTTTTTcgaaaattgt
Reference(s): [3] Kaczanowska M., et al., 2004
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP repressor rplMp 3378850 3378866 -185.5 gggttaaaacCTGTCACAAATCACAAAaaggggtcga nd [AIBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd FNR1 repressor rplMp nd nd nd nd nd [AIBSCS], [CV(GEA/ROMA)], [GEA] [6]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis repressor rplMp 3378679 3378699 -17.0 acaattgaagACGTTTGGGTGTTCACCAACGtgtaactatt nd [AIBSCS], [APIORCISFBSCS] [5]
Note(s): 1FNR represses the rplM-rps operon expression under anaerobiosis. A putative FNR-binding site, which was not shown in the paper, was identified upstream of this operon Salmon K,2003.3FNR represses the rplM-rps operon expression under anaerobiosis. A putative FNR-binding site, which was not shown in the paper, was identified upstream of this operon Salmon K,2003.
Allosteric regulation of RNA-polymerase
  Regulator Function Promoter target of RNApol Growth Conditions Note Evidence Reference
  DksA-ppGpp inhibition rplMp The rplM-rpsI operon is regulated by ppGpp-DksA-dependent negative stringent control under amino acid starvation, most likely due to the features of the discriminator region (G/C rich) separating the -10 promoter element from the transcription start site (TSS) Aseev LV,2016 [GEA] [7]
Evidence: [GEA] Gene expression analysis
Reference(s): [7] Aseev LV., et al., 2016


Transcription unit       
Gene(s): rpsI   Genome Browser M3D Gene expression COLOMBOS
Evidence: [BTEI] Boundaries of transcription experimentally identified
[IC] Inferred by curator
Reference(s): [1] Huerta AM., et al., 2003
[3] Kaczanowska M., et al., 2004
Terminator(s)
Type: rho-independent
Sequence: ttttagctatAAAAAAACCCGCCGAAGCGGGTTTTTTcgaaaattgt
Reference(s): [3] Kaczanowska M., et al., 2004





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