RegulonDB RegulonDB 11.2: Operon Form
   

xseB-ispA-dxs-yajO operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: xseB-ispA-dxs-yajO
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: xseB-ispA-dxs-yajO
Gene(s): yajO, dxs, ispA, xseB   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-TRANSCRIPT-LEN-DETERMINATION] Length of transcript experimentally determined
Reference(s): [1] Fujisaki S., et al., 1990
[2] Lois LM., et al., 1998
[3] Vales LD., et al., 1982
[4] Vales LD., et al., 1983
Promoter
Name: xseBp
+1: 441373
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 30
Sequence: cacatcaccttaccattgcgcgttatttgctatttgccctgagtccgttaccatgacgggGcgaaaaatattgagagtcag
                                -35                   -10   +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
Reference(s): [1] Fujisaki S., et al., 1990
[5] Huerta AM., et al., 2003


Transcription unit       
Name: yajO
Gene(s): yajO   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Reference(s): [6] Zaslaver A., et al., 2006
Promoter
Name: yajOp
+1: Unknown
Evidence: [EXP-IEP]
Reference(s): [6] Zaslaver A., et al., 2006


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IEP] Inferred from expression pattern


Reference(s)    

 [1] Fujisaki S., Hara H., Nishimura Y., Horiuchi K., Nishino T., 1990, Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli., J Biochem 108(6):995-1000

 [2] Lois LM., Campos N., Putra SR., Danielsen K., Rohmer M., Boronat A., 1998, Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis., Proc Natl Acad Sci U S A 95(5):2105-10

 [3] Vales LD., Rabin BA., Chase JW., 1982, Subunit structure of Escherichia coli exonuclease VII., J Biol Chem 257(15):8799-805

 [4] Vales LD., Rabin BA., Chase JW., 1983, Isolation and preliminary characterization of Escherichia coli mutants deficient in exonuclease VII., J Bacteriol 155(3):1116-22

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [6] Zaslaver A., Bren A., Ronen M., Itzkovitz S., Kikoin I., Shavit S., Liebermeister W., Surette MG., Alon U., 2006, A comprehensive library of fluorescent transcriptional reporters for Escherichia coli., Nat Methods 3(8):623-8

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