RegulonDB RegulonDB 10.9: Operon Form
   

htpX operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: htpX
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit       
Name: htpX
Gene(s): htpX   Genome Browser M3D Gene expression COLOMBOS
Note(s): A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for htpX . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733.
Evidence: [BTEI] Boundaries of transcription experimentally identified
Reference(s): [1] Kornitzer D., et al., 1991
Promoter
Name: htpXp
+1: 1912618
Sigma Factor: Sigma32 Sigmulon
Distance from start of the gene: 42
Sequence: tctggtgacttacgcactatccagacttgaaaatagtcgcgtaacccatacgatgtgggtAtcgcatattgcgttttgtta
                           -35              -10             +1                   
Note(s): The distance between the -10 region and the -35 region (12 nucleotides) is slightly shorter than the spacing in the other heat shock promoters (13-15 nucleotides), whereas the distance between the -10 region and the transcription start site is 1-2 nucleotides longer than this distance in the other heat shock promoters.
Evidence: [AIPP]
[HIPP]
[ICWHO]
[IDA]
[IEP]
[RS-EPT-CBR]
[TIM]
Reference(s): [2] Huerta AM., et al., 2003
[1] Kornitzer D., et al., 1991
[3] Nonaka G., et al., 2006
[4] Salgado H, et al., 2012
[5] Wade JT., et al., 2006
Terminator(s)
Type: rho-independent
Sequence: gggcataaaaAAAGCGCGTCGATCAGGACGCGCTTTTTagtatttact
Reference(s): [1] Kornitzer D., et al., 1991


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Transcriptional
Strand: reverse
Evidence: [ICA] Inferred by computational analysis
Reference(s): [6] Merino E, et al., 2005
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -18.7 1912731 1912762 agtaatatcaATCAGGCACAAGAAATTGTGCCTGATTTTTTaacagcgaca
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"




Reference(s)    

 [1] Kornitzer D., Teff D., Altuvia S., Oppenheim AB., 1991, Isolation, characterization, and sequence of an Escherichia coli heat shock gene, htpX., J Bacteriol 173(9):2944-53

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Nonaka G., Blankschien M., Herman C., Gross CA., Rhodius VA., 2006, Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress., Genes Dev 20(13):1776-89

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Wade JT., Roa DC., Grainger DC., Hurd D., Busby SJ., Struhl K., Nudler E., 2006, Extensive functional overlap between sigma factors in Escherichia coli., Nat Struct Mol Biol 13(9):806-14

 [6] Merino E, Yanofsky C., 2005, Transcription attenuation: a highly conserved regulatory strategy used by bacteria., Trends Genet. 2005 May;21(5):260-4.


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