RegulonDB RegulonDB 10.9: Operon Form

grpE operon and associated TUs in Escherichia coli K-12 genome

Name: grpE
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: grpE
Gene(s): grpE   Genome Browser M3D Gene expression COLOMBOS
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Lipinska B., et al., 1988
Name: grpEp
+1: 2750747
Sigma Factor: Sigma32 Sigmulon
Distance from start of the gene: 39
Sequence: gattgatgacaatgtgagtgcttcccttgaaaccctgaaactgatccccataataagcgaAgttagcgagatgaatgcgaa
                           -35                -10           +1                   
Note(s): There is the possibility that this gene may also be transcribed by the RNA polymerase σ70 holoenzyme.
Evidence: [AIPP]
Reference(s): [2] Huerta AM., et al., 2003
[1] Lipinska B., et al., 1988
[3] Nonaka G., et al., 2006
[4] Salgado H, et al., 2012
[5] Wade JT., et al., 2006
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cAMP activator grpEp 2750980 2751002 -243.5 ccaatctccgCGAGCGTGCCAGTTTTCACATTCttcagttgca nd [AIBSCS], [GEA] [6], [7]


 [1] Lipinska B., King J., Ang D., Georgopoulos C., 1988, Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein., Nucleic Acids Res 16(15):7545-62

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Nonaka G., Blankschien M., Herman C., Gross CA., Rhodius VA., 2006, Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress., Genes Dev 20(13):1776-89

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Wade JT., Roa DC., Grainger DC., Hurd D., Busby SJ., Struhl K., Nudler E., 2006, Extensive functional overlap between sigma factors in Escherichia coli., Nat Struct Mol Biol 13(9):806-14

 [6] Tsai MJ., Wang JR., Yang CD., Kao KC., Huang WL., Huang HY., Tseng CP., Huang HD., Ho SY., 2018, PredCRP: predicting and analysing the regulatory roles of CRP from its binding sites in Escherichia coli., Sci Rep 8(1):951

 [7] Zheng D., Constantinidou C., Hobman JL., Minchin SD., 2004, Identification of the CRP regulon using in vitro and in vivo transcriptional profiling., Nucleic Acids Res 32(19):5874-93