RegulonDB RegulonDB 10.9: Operon Form

upp-uraA operon and associated TUs in Escherichia coli K-12 genome

Name: upp-uraA
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: upp-uraA
Gene(s): uraA, upp   Genome Browser M3D Gene expression COLOMBOS
Note(s): This operon is regulated by pyrimidine-mediated reiterative transcription. There is a putative internal terminator between upp and uraA.
The intergenic region of upp-purMN protects against bacterial cell death via both type I and type II covalent topoisomerase-DNA cleavage complexes Liu IF,2011 Deletion of the binding site for FNR and PurR from the intergenic region of upp-purMN decreases the protective effect, inducing lethality via titration Liu IF,2011
Evidence: [BTEI] Boundaries of transcription experimentally identified
[PM] Polar mutation
Reference(s): [1] Andersen PS., et al., 1995
[2] Tu AH., et al., 1997
Name: uppp
+1: 2620909
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 37
Sequence: ttttgactaaagtcaacgaaaagaatattgccgccttgaagaaaggaggtataatccgtcGattttttttgtggctgcccc
                            -35                   -10       +1                   
Note(s): The initially transcribed upp region contains the sequence GATTTTTTTTG. Regulation involving reiterative transcription appears to be completely eliminated when the T/A tract contains three or fewer base pairs.
Evidence: [HIPP]
Reference(s): [3] Andersen PS., et al., 1992
[4] Cheng Y., et al., 2001
[5] Huerta AM., et al., 2003
[2] Tu AH., et al., 1997
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FNR activator uppp 2620964 2620977 -61.5 ttatctcaaaCCGTTATCATTTTGActaaagtcaa nd [AIBSCS], [GEA] [6]