RegulonDB RegulonDB 10.9: Operon Form
   

fecIR operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: fecIR
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: fecIR
Gene(s): fecR, fecI   Genome Browser M3D Gene expression COLOMBOS
Note(s): Previous information suggested only one transcription unit, fecIRABCDE. Current information suggests that there are two transcription units, fecIR and fecABCDE.
A possible role in the rpsA-ihfB, adhP, fecIR, and fecABCDE operons was described for functional abortive RNAs (faRNAs), based on computational prediction and described by Nair et al. 2017 28347827
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Arechaga I., et al., 2003
[2] Enz S., et al., 1995
Promoter
Name: fecIp
+1: 4518282
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 47
Sequence: acaacatgttaaaaatgtctattggaaacaattttatttccaattgtaatgataaccattCtcatattaatatgactacgt
                      -35                       -10         +1                   
Evidence: [CV(RPF/TIM)]
[HIPP]
[ICWHO]
[RPF]
[TIM]
Reference(s): [2] Enz S., et al., 1995
[3] Huerta AM., et al., 2003
[4] Ochs M., et al., 1996
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fur-Fe2+ repressor fecIp 4518277 4518295 -4.0 tttccaattgTAATGATAACCATTCTCATAttaatatgac nd [APIORCISFBSCS], [BPP] [7]
proximal Fur-Fe2+ repressor fecIp 4518280 4518298 -7.0 ttatttccaaTTGTAATGATAACCATTCTCatattaatat nd [AIBSCS], [BPP], [GEA], [SM] [1], [7], [9]
proximal Fur-Fe2+ repressor fecIp 4518283 4518301 -10.0 attttatttcCAATTGTAATGATAACCATTctcatattaa nd [APIORCISFBSCS], [BPP] [7]
proximal Fur-Fe2+ repressor fecIp 4518287 4518305 -14.0 aacaattttaTTTCCAATTGTAATGATAACcattctcata nd [APIORCISFBSCS], [BPP] [1], [6], [7], [8]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal SlyA repressor fecIp 4518345 4518344 -62.5 aacacttagaAaaacaacatg nd [AIBSCS], [BPP], [GEA] [5]




Reference(s)    

 [1] Arechaga I., Miroux B., Runswick MJ., Walker JE., 2003, Over-expression of Escherichia coli F1F(o)-ATPase subunit a is inhibited by instability of the uncB gene transcript., FEBS Lett 547(1-3):97-100

 [2] Enz S., Braun V., Crosa JH., 1995, Transcription of the region encoding the ferric dicitrate-transport system in Escherichia coli: similarity between promoters for fecA and for extracytoplasmic function sigma factors., Gene 163(1):13-8

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Ochs M., Angerer A., Enz S., Braun V., 1996, Surface signaling in transcriptional regulation of the ferric citrate transport system of Escherichia coli: mutational analysis of the alternative sigma factor FecI supports its essential role in fec transport gene transcription., Mol Gen Genet 250(4):455-65

 [5] Curran TD., Abacha F., Hibberd SP., Rolfe MD., Lacey MM., Green J., 2017, Identification of new members of the Escherichia coli K-12 MG1655 SlyA regulon., Microbiology 163(3):400-409

 [6] Angerer A., Braun V., 1998, Iron regulates transcription of the Escherichia coli ferric citrate transport genes directly and through the transcription initiation proteins., Arch Microbiol 169(6):483-90

 [7] Chen Z., Lewis KA., Shultzaberger RK., Lyakhov IG., Zheng M., Doan B., Storz G., Schneider TD., 2007, Discovery of Fur binding site clusters in Escherichia coli by information theory models., Nucleic Acids Res 35(20):6762-77

 [8] Pressler U., Staudenmaier H., Zimmermann L., Braun V., 1988, Genetics of the iron dicitrate transport system of Escherichia coli., J Bacteriol 170(6):2716-24

 [9] Stiefel A., Mahren S., Ochs M., Schindler PT., Enz S., Braun V., 2001, Control of the ferric citrate transport system of Escherichia coli: mutations in region 2.1 of the FecI extracytoplasmic-function sigma factor suppress mutations in the FecR transmembrane regulatory protein., J Bacteriol 183(1):162-70


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