RegulonDB RegulonDB 10.9: Operon Form
   

fruBKA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: fruBKA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: fruBKA
Synonym(s): OP00220, fruBMH
Gene(s): fruA, fruK, fruB   Genome Browser M3D Gene expression COLOMBOS
Note(s): A potential RNA G-quadruplex structure, formed by guanine-rich sequences located in the coding sequence region of the gene, was identified for fruA . This structure could regulate the expression of the gene, as observed for hemL gene expression 31964733.
Evidence: [TAS] Traceable author statement
Reference(s): [1] Dobrynina OIu., et al., 1995
[2] Reizer J., et al., 1994
Promoter
Name: fruBp
+1: 2263597
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 102
Sequence: aaacttttgctcataactttacggctttccttgcgtgctgaaacagaagtattatgctttCttgaaacgtttcagcgcgat
                          -35                     -10       +1                   
Evidence: [AIPP]
[ICWHO]
Reference(s): [3] Huerta AM., et al., 2003
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote Cra repressor fruBp 2263516 2263533 73.5 tttttctcatAGAGGCTGAATCGTTTCAAttcagcaaga nd [AIBSCS], [GEA] [5]
proximal Cra2 repressor fruBp 2263582 2263599 7.5 agtattatgcTTTCTTGAAACGTTTCAGCgcgatcttgt nd [AIBSCS], [GEA], [NTAS] [4], [5]
Note(s): 2Even though the studies of FruR regulation of fruBKA were done in Salmonella enterica serovar Typhimurium, the inferences can be applied to E. coli also Ramseier TM,1993. It was observed that in presence of fructose-1-phosphate FruR cannot bind to its fruB site, allowing its transcription. In addition, two FruR operators have been observed in the regulatory region of fruBKA, so a repression mechanism of FruR for this operon has been proposed: as the two operators are distant, FruR binds to both sites and bends the DNA, avoiding the RNA polymerase access to the promoter. For a discussion of the serovar Typhimurium evidence see Ramseier TM,1993.1Even though the studies of FruR regulation of fruBKA were done in Salmonella enterica serovar Typhimurium, the inferences can be applied to E. coli also Ramseier TM,1993. It was observed that in presence of fructose-1-phosphate FruR cannot bind to its fruB site, allowing its transcription. In addition, two FruR operators have been observed in the regulatory region of fruBKA, so a repression mechanism of FruR for this operon has been proposed: as the two operators are distant, FruR binds to both sites and bends the DNA, avoiding the RNA polymerase access to the promoter. For a discussion of the serovar Typhimurium evidence see Ramseier TM,1993.


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: reverse
Evidence: [ICA] Inferred by computational analysis
Reference(s): [6] Merino E, et al., 2005
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -12.52 2263498 2263541 ggcgctttttTTCTCATAGAGGCTGAATCGTTTCAATTCAGCAAGAGAGGAGAactatgttcc
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"





RegulonDB