RegulonDB RegulonDB 10.8: Operon Form

fepA-entD operon and associated TUs in Escherichia coli K-12 genome

Name: fepA-entD
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: fepA-entD
Synonym(s): OP00224, fepA
Gene(s): entD, fepA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Expression of fepA, along with that of other genes also regulated by Fur, is reduced under Zn excess Xu Z,2019.
Reference(s): [1] Hunt MD., et al., 1994
Name: fepAp
+1: 612667
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 173
Sequence: ccatgtttactgtgcaatttttcattgattgcagaaatatattgataatattattgataaCtatttgcatttgcaatagcg
                         -35                     -10        +1                   
Evidence: [TIM]
Reference(s): [2] Escolar L., et al., 1998
[3] Pettis GS., et al., 1988
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator fepAp 612699 612720 -42.5 accccatgttTACTGTGCAATTTTTCATTGATtgcagaaata nd [AIBSCS], [CV(GEA/ROMA)], [GEA] [4]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fur-Fe2+ repressor fepAp 612650 612668 9.0 attattgataACTATTTGCATTTGCAATAgcgtaatggc nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [1], [4], [5], [6], [7], [8]
proximal Fur-Fe2+ repressor fepAp 612657 612675 2.0 tgataatattATTGATAACTATTTGCATTtgcaatagcg nd [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [4], [5], [6], [7]
proximal Fur-Fe2+ repressor fepAp 612663 612681 -5.0 atatattgatAATATTATTGATAACTATTtgcatttgca nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [4], [5], [6], [7]
proximal Fur-Fe2+ repressor fepAp 612669 612687 -11.0 gcagaaatatATTGATAATATTATTGATAactatttgca nd [AIBSCS], [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [4], [5], [6], [7]
proximal Fur-Fe2+ repressor fepAp 612676 612694 -18.0 attgattgcaGAAATATATTGATAATATTattgataact nd [APIORCISFBSCS], [BPP], [CV(CHIP-SV/GEA/ROMA)], [CV(GEA/ROMA)], [GEA] [5], [6], [7]

Regulation by sRNA    
  Small RNA name (Regulator) Regulation type Mechanism Function Binding Sites Evidence Reference
LeftPos RightPos Sequence (RNA-strand)
  omrA antisense post-transcriptional regulation repressor       [IMP] [9]
Notes: "The provided sequence is that of the RNA strand,i.e. 'U's are showed instead the 'T'"

RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: reverse
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -6.3 612514 612538 atgacgaccaTGCCCGACAGTTGCAATTCGTGGCaaaaatgcag
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


 [1] Hunt MD., Pettis GS., McIntosh MA., 1994, Promoter and operator determinants for fur-mediated iron regulation in the bidirectional fepA-fes control region of the Escherichia coli enterobactin gene system., J Bacteriol 176(13):3944-55

 [2] Escolar L., Perez-Martin J., de Lorenzo V., 1998, Coordinated repression in vitro of the divergent fepA-fes promoters of Escherichia coli by the iron uptake regulation (Fur) protein., J Bacteriol 180(9):2579-82

 [3] Pettis GS., Brickman TJ., McIntosh MA., 1988, Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter., J Biol Chem 263(35):18857-63

 [4] Zhang Z., Gosset G., Barabote R., Gonzalez CS., Cuevas WA., Saier MH., 2005, Functional interactions between the carbon and iron utilization regulators, Crp and Fur, in Escherichia coli., J Bacteriol 187(3):980-90

 [5] Chen Z., Lewis KA., Shultzaberger RK., Lyakhov IG., Zheng M., Doan B., Storz G., Schneider TD., 2007, Discovery of Fur binding site clusters in Escherichia coli by information theory models., Nucleic Acids Res 35(20):6762-77

 [6] Kumar R., Shimizu K., 2011, Transcriptional regulation of main metabolic pathways of cyoA, cydB, fnr, and fur gene knockout Escherichia coli in C-limited and N-limited aerobic continuous cultures., Microb Cell Fact 10:3

 [7] Newman DL., Shapiro JA., 1999, Differential fiu-lacZ fusion regulation linked to Escherichia coli colony development., Mol Microbiol 33(1):18-32

 [8] Vassinova N., Kozyrev D., 2000, A method for direct cloning of fur-regulated genes: identification of seven new fur-regulated loci in Escherichia coli., Microbiology 146 Pt 12:3171-3182

 [9] Guillier M., Gottesman S., 2006, Remodelling of the Escherichia coli outer membrane by two small regulatory RNAs., Mol Microbiol 59(1):231-47