RegulonDB RegulonDB 10.9: Operon Form
   

mglBAC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: mglBAC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: mglAC
Gene(s): mglC, mglA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [ITCR] Inferred through co-regulation
Reference(s): [1] Harayama S., et al., 1983
Promoter
Name: mglAp
+1: 2239391
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 102
Sequence: ggtgcggctgatggcaccaactggaaaatcgacaacaaagtggtccgcgtaccttatgttGgcgtagataaagacaacctg
                            -35                        -10  +1                   
Evidence: [AIPP]
[ICWHO]
Reference(s): [2] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[Fis,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd Fis1 repressor mglAp nd nd nd nd nd [BPP], [GEA], [IGI] [3]
Note(s): 1Both Fis and RpoS negatively regulated mglA in a gene expression analysis. The RpoS effect could be indirect Xu J,1995.1Both Fis and RpoS negatively regulated mglA in a gene expression analysis. The RpoS effect could be indirect Xu J,1995.


Transcription unit          
Name: mglBAC
Synonym(s): OP00230, mglBAK
Gene(s): mglC, mglA, mglB   Genome Browser M3D Gene expression COLOMBOS
Note(s): Under NlpE outer membrane lipoprotein overexpression, the transcription of the mglB gene is decreased Raivio TL,2013 mglBAC, among other genes involved in carbon source transport and metabolism, were downregulated in two MG1655 lysogens carrying closely related Stx2a phages O104 and PA8 1208335|.
A transcript that contains mglB and mglA is decreased after σE induction, as observed in high-throughput analysis of gene expression Lacoux C,2020.
Evidence: [ITCR] Inferred through co-regulation
[PM] Polar mutation
Reference(s): [1] Harayama S., et al., 1983
[4] Hogg RW., et al., 1991
Promoter
Name: mglBp2
+1: 2240566
Sigma Factor: Sigma38 Sigmulon
Distance from start of the gene: 218
Sequence: cgctttcaatctgtgagtgatttcacagtatcttaacaatgtgatagctatgattgcaccGttttaacgttgtaacccgta
                        -35                 -10             +1                   
Evidence: [HIPP]
[ICWHO]
[IMP]
Reference(s): [4] Hogg RW., et al., 1991
[2] Huerta AM., et al., 2003
[5] Notley-McRobb L., et al., 2002
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator mglBp2 2240597 2240618 -41.5 aaccgctttcAATCTGTGAGTGATTTCACAGTAtcttaacaat nd [AIBSCS], [BCE], [BPP], [GEA], [SM] [7], [8], [9], [10]
[FlhDC,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd FlhDC1 repressor mglBp2 nd nd nd nd nd [BPP], [GEA], [IGI] [11]
[GalR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GalR repressor mglBp2 2240618 2240633 -59.5 caaatcaggcGATGTAACCGCTTTCAAtctgtgagtg nd [APIORCISFBSCS], [GEA] [6]
[GalS,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GalS repressor mglBp2 2240618 2240633 -59.5 caaatcaggcGATGTAACCGCTTTCAAtctgtgagtg nd [APIORCISFBSCS], [GEA] [6]
Note(s): 1The regulatory effect of FlhDC in mglB operon transcription has been proved by microarray analysis by Pruss BM,20014The regulatory effect of FlhDC in mglB operon transcription has been proved by microarray analysis by Pruss BM,2001


Transcription unit          
Name: mglBAC
Synonym(s): OP00230, mglBAK
Gene(s): mglC, mglA, mglB   Genome Browser M3D Gene expression COLOMBOS
Note(s): Under NlpE outer membrane lipoprotein overexpression, the transcription of the mglB gene is decreased Raivio TL,2013 mglBAC, among other genes involved in carbon source transport and metabolism, were downregulated in two MG1655 lysogens carrying closely related Stx2a phages O104 and PA8 1208335|.
A transcript that contains mglB and mglA is decreased after σE induction, as observed in high-throughput analysis of gene expression Lacoux C,2020.
Evidence: [ITCR] Inferred through co-regulation
[PM] Polar mutation
Reference(s): [1] Harayama S., et al., 1983
[4] Hogg RW., et al., 1991
Promoter
Name: mglBp
+1: 2240566
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 218
Sequence: cgctttcaatctgtgagtgatttcacagtatcttaacaatgtgatagctatgattgcaccGttttaacgttgtaacccgta
                      -35                        -10        +1                   
Evidence: [HIPP]
[ICWHO]
[IMP]
Reference(s): [4] Hogg RW., et al., 1991
[2] Huerta AM., et al., 2003
[5] Notley-McRobb L., et al., 2002
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator mglBp 2240597 2240618 -41.5 aaccgctttcAATCTGTGAGTGATTTCACAGTAtcttaacaat nd [AIBSCS], [BCE], [BPP], [GEA], [SM] [7], [8], [9], [10]
[FlhDC,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd FlhDC1 repressor mglBp nd nd nd nd nd [BPP], [GEA], [IGI] [11]
[GalR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GalR repressor mglBp 2240618 2240633 -59.5 caaatcaggcGATGTAACCGCTTTCAAtctgtgagtg nd [APIORCISFBSCS], [GEA] [6]
[GalS,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal GalS repressor mglBp 2240618 2240633 -59.5 caaatcaggcGATGTAACCGCTTTCAAtctgtgagtg nd [APIORCISFBSCS], [GEA] [6]
Note(s): 1The regulatory effect of FlhDC in mglB operon transcription has been proved by microarray analysis by Pruss BM,20014The regulatory effect of FlhDC in mglB operon transcription has been proved by microarray analysis by Pruss BM,2001


Evidence    

 [AIPP] Automated inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [BPP] Binding of purified proteins

 [GEA] Gene expression analysis

 [IGI] Inferred from genetic interaction

 [HIPP] Human inference of promoter position

 [IMP] Inferred from mutant phenotype

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BCE] Binding of cellular extracts

 [SM] Site mutation

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.


Reference(s)    

 [1] Harayama S., Bollinger J., Iino T., Hazelbauer GL., 1983, Characterization of the mgl operon of Escherichia coli by transposon mutagenesis and molecular cloning., J Bacteriol 153(1):408-15

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Xu J., Johnson RC., 1995, Identification of genes negatively regulated by Fis: Fis and RpoS comodulate growth-phase-dependent gene expression in Escherichia coli., J Bacteriol 177(4):938-47

 [4] Hogg RW., Voelker C., Von Carlowitz I., 1991, Nucleotide sequence and analysis of the mgl operon of Escherichia coli K12., Mol Gen Genet 229(3):453-9

 [5] Notley-McRobb L., King T., Ferenci T., 2002, rpoS mutations and loss of general stress resistance in Escherichia coli populations as a consequence of conflict between competing stress responses., J Bacteriol 184(3):806-11

 [6] Geanacopoulos M., Adhya S., 1997, Functional characterization of roles of GalR and GalS as regulators of the gal regulon., J Bacteriol 179(1):228-34

 [7] Hollands K., Busby SJ., Lloyd GS., 2007, New targets for the cyclic AMP receptor protein in the Escherichia coli K-12 genome., FEMS Microbiol Lett 274(1):89-94

 [8] Jackson RJ., Binet MR., Lee LJ., Ma R., Graham AI., McLeod CW., Poole RK., 2008, Expression of the PitA phosphate/metal transporter of Escherichia coli is responsive to zinc and inorganic phosphate levels., FEMS Microbiol Lett 289(2):219-24

 [9] Weickert MJ., Adhya S., 1993, Control of transcription of gal repressor and isorepressor genes in Escherichia coli., J Bacteriol 175(1):251-8

 [10] Zheng D., Constantinidou C., Hobman JL., Minchin SD., 2004, Identification of the CRP regulon using in vitro and in vivo transcriptional profiling., Nucleic Acids Res 32(19):5874-93

 [11] Pruss BM., Liu X., Hendrickson W., Matsumura P., 2001, FlhD/FlhC-regulated promoters analyzed by gene array and lacZ gene fusions., FEMS Microbiol Lett 197(1):91-7

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