RegulonDB RegulonDB 10.10: Operon Form
   

umuDC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: umuDC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: umuDC
Synonym(s): OP00250
Gene(s): umuD, umuC   Genome Browser M3D Gene expression COLOMBOS
Note(s): umuDC, among other genes that form the SOS response regulon, belong to the most abundant class of upregulated genes in two MG1655 lysogens carrying closely related Stx2a phages O104 and PA8 1208335|.
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Fernandez De Henestrosa AR., et al., 2000
[2] Opperman T., et al., 1996
Promoter
Name: umuDp
+1: 1230738
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 29
Sequence: gacaaatattgatagcctgaatcagtattgatctgctggcaagaacagactactgtatatAaaaacagtataacttcaggc
                            -35                    -10      +1                   
Evidence: [HIPP]
[ICWHO]
[TIM]
Reference(s): [3] Huerta AM., et al., 2003
[4] Kitagawa Y., et al., 1985
TF binding sites (TFBSs)
[LexA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal LexA repressor umuDp 1230708 1230727 -20.5 atcagtattgATCTGCTGGCAAGAACAGACtactgtatat nd [APIORCISFBSCS] [4], [5]
proximal LexA repressor umuDp 1230728 1230747 -0.5 aagaacagacTACTGTATATAAAAACAGTAtaacttcagg nd [BPP] [4], [5]


Evidence    

 [HIPP] Human inference of promoter position

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [BPP] Binding of purified proteins


Reference(s)    

 [1] Fernandez De Henestrosa AR., Ogi T., Aoyagi S., Chafin D., Hayes JJ., Ohmori H., Woodgate R., 2000, Identification of additional genes belonging to the LexA regulon in Escherichia coli., Mol Microbiol 35(6):1560-72

 [2] Opperman T., Murli S., Walker GC., 1996, The genetic requirements for UmuDC-mediated cold sensitivity are distinct from those for SOS mutagenesis., J Bacteriol 178(15):4400-11

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Kitagawa Y., Akaboshi E., Shinagawa H., Horii T., Ogawa H., Kato T., 1985, Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli., Proc Natl Acad Sci U S A 82(13):4336-40

 [5] Lewis LK., Harlow GR., Gregg-Jolly LA., Mount DW., 1994, Identification of high affinity binding sites for LexA which define new DNA damage-inducible genes in Escherichia coli., J Mol Biol 241(4):507-23

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