RegulonDB RegulonDB 10.9: Operon Form
   

glyA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: glyA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: glyA
Synonym(s): OP00267
Gene(s): glyA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Lorenz et al. in 1996 showed that MetR at low concentrations represses the glyA gene, when it binds on the binding site located at -142 Lorenz E,1996, and simultaneously activates the transcription of the hmp gene Membrillo-Hernandez J,1998. In addition, MetR functions as an activator when it binds as a dimer of dimers to binding sites located at -121 and -143 relative to the transcription start site of glyA.
Based on gene expression analysis, it was shown that CsgD could positively regulate glyA expression Herrington MB,2013
Reference(s): [1] Lorenz E., et al., 1995
[2] Steiert JG., et al., 1992
Promoter
Name: glyAp
+1: 2685574
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 67
Sequence: cgtttgcgtaaattcctttgtcaagacctgttatcgcacaatgattcggttatactgttcGccgttgtccaacaggaccgc
                            -35                    -10      +1                   
Evidence: [CV(RPF/TIM)]
[HIPP]
[ICWHO]
[RPF]
[TIM]
Reference(s): [3] Huerta AM., et al., 2003
[4] Plamann MD., et al., 1983
Terminator(s)
Type: rho-independent
Sequence: taacgtagaaAGGCTTCCCGAAGGAAGCCttgatgatca
Reference(s): [5] Plamann MD., et al., 1983
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MetR-L-homocysteine activator glyAp 2685688 2685702 -121.0 gcatcacaaaCCTGAAAAGAAATCCGtttccggttg nd [APIORCISFBSCS], [BPP], [GEA], [SM] [1], [6], [7], [8]
remote MetR-L-homocysteine activator glyAp 2685710 2685724 -143.0 aatgtgatgaAGTGAAAAATTTGCATcacaaacctg nd [APIORCISFBSCS], [BPP], [GEA], [IMP], [SM] [1], [6], [7], [8]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote MetR-L-homocysteine repressor glyAp 2685710 2685724 -143.0 aatgtgatgaAGTGAAAAATTTGCATcacaaacctg nd [APIORCISFBSCS], [BPP], [GEA], [IMP], [SM] [1], [6], [7], [8]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal PurR-hypoxanthine repressor glyAp 2685626 2685641 -59.5 gcgtagcctgAAGGTAATCGTTTGCGTaaattccttt nd [AIBSCS], [APIORCISFBSCS], [BPP], [GEA], [SM] [1], [2], [6], [9]





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